@article { , title = {Biological activity and stability of aeruginosamides from cyanobacteria.}, abstract = {Aeruginosamides (AEGs) are classified as cyanobactins, ribosomally synthesized peptides with post-translational modifications. They have been identified in cyanobacteria of genera Microcystis, Oscillatoria, and Limnoraphis. In this work, the new data on the in vitro activities of three AEG variants, AEG A, AEG625 and AEG657, and their interactions with metabolic enzymes are reported. Two aeruginosamides, AEG625 and AEG657, decreased the viability of human breast cancer cell line T47D, but neither of the peptides was active against human liver cancer cell line Huh7. AEGs also did not change the expression of MIR92b-3p, but for AEG625, the induction of oxidative stress was observed. In the presence of a liver S9 fraction containing microsomal and cytosolic enzymes, AEG625 and AEG657 showed high stability. In the same assays, quick removal of AEG A was recorded. The peptides had mild activity against three cytochrome P450 enzymes, CYP2C9, CYP2D6 and CYP3A4, but only at the highest concentration used in the study (60 µM). The properties of AEGs, i.e., cytotoxic activity and in vitro interactions with important metabolic enzymes, form a good basis for further studies on their pharmacological potential.}, doi = {10.3390/md20020093}, eissn = {1660-3397}, issue = {2}, journal = {Marine Drugs}, note = {INFO COMPLETE (Info via contact 21/1/2022 LM) PERMISSION GRANTED (version = VOR; embargo = none; licence = BY; SHERPA = https://v2.sherpa.ac.uk/id/publication/17519 ) DOCUMENT READY (VOR downloaded 21/1/2022 LM) ADDITIONAL INFO - Contact: Christine Edwards}, publicationstatus = {Published}, publisher = {MDPI}, url = {https://rgu-repository.worktribe.com/output/1575515}, volume = {20}, keyword = {Environment, Energy & Sustainability, Cyanopeptides, Aeruginosamides, Cytotoxicity, MiRNA, Cytochrome P450 enzymes, Metabolic stability}, year = {2022}, author = {Ceg?owska, Marta and Kwiecie?, Patrycja and Szubert, Karolina and Brzuzan, Pawe? and Florczyk, Maciej and Edwards, Christine and Kosakowska, Alicja and Mazur-Marzec, Hanna} }