@inproceedings { , title = {Characterisation of peroxidase-like activity of thermally synthesized gold nanoparticles.}, abstract = {The development of nanotechnologies has attracted a lot of interest since it brings new possibilities in the field of nanomedical applications, from diagnostic methods to targeted nanotransporters. Gold nanoparticles (AuNPs) belong to a group of nanomaterials with significant peroxidase-like activity. We have successfully synthesized AuNPs thermally over a range of temperatures from 20 to 100°C. The AuNPs size varied from 17 to 37 nm and the zeta potential was found to vary from-32 to-44 mV, in which the maximum absorption maximum was detected at 529 nm. Peroxidase-like activity was monitored in the presence of TMB (1 mM) and hydrogen peroxide (10 mM). The proposed system based on peroxidase-like activity was further evaluated in immune detection of human IgG, in which anti-IgG antibody was modified with AuNPs.}, conference = {8th Nanomaterials international conference 2016 (NANOCON 2016): research and application}, note = {COMPLETED PERMISSION GRANTED (version = VOR; embargo = none; licence = BY; POLICY = https://www.confer.cz/about 17/12/2020 LM) DOCUMENT READY (VOR downloaded 17/12/2020 LM) ADDITIONAL INFO - Contact: Carlos Fernandez}, pages = {429-434}, publicationstatus = {Published}, publisher = {Tanger Ltd.}, url = {https://rgu-repository.worktribe.com/output/247442}, keyword = {Gold nanoparticles, electrochemistry, spectrophotometry, enzymatic reaction, ELISA, nanomedicine}, year = {2017}, author = {Docekalova, Michaela and Uhlirova, Dagmar and Stankova, Martina and Kepinska, Marta and Sochor, Jiri and Milnerowicz, Halina and Babula, Petr and Fernandez, Carlos and Brazdova, Marie and Zidkova, Jarmila and Suchy, Pavel and Kizek, Rene} }