@article { , title = {Phosphate and nitrate supplementations to evaluate the effect on cell biomass, intra and extracellular nodularin and nodulopeptin 901 produced by the cyanobacterium Nodularia spumigena KAC 66.}, abstract = {Blooms of the cyanobacterium Nodularia spumigena occur regularly in the Baltic Sea typically producing a wide range of bioactive peptides including the hepatotoxin nodularin (NOD), spumigins, anabaenopeptins and nodulopeptins (molecular weights: 917, 901 and 899Da). This study reports the production of intracellular and extracellular NOD and nodulopeptin 901 (the major secondary metabolites), at various nitrate and phosphorus concentrations produced by N. spumigena KAC 66 which had been originally isolated from the Baltic Sea. The growth was observed by cell biomass and intracellular and extracellular peptides monitored by high-performance liquid chromatography with photodiode array and mass spectrometry (HPLC-PDA-MS). In the present work, it was found that high concentrations of nitrate and phosphorus have a considerable effect on biomass and toxin levels of N. spumigena. In common with many studies, the maximum amount of NOD was retained within the cells during 5weeks of growth. In contrast, as much as ~ 40\% of nodulopeptin 901 was excreted into the medium throughout the duration of experiments. At 6.5 and 3.5mgL−1 nitrate, the maximum concentrations of peptide per unit biomass was 1.78ng NOD (in week 4) and 1.42ngnodulopeptin 901 μg−1 (in week 3) were detected. However, the high concentrations of both peptides were produced in the absence of nitrate. The phosphate experiment indicated growth, and peptide production was dependent on availability of phosphorus. At 0mgL−1 of phosphate, an increased amount of intracellular (502.4ngμg−1 biomass) nodulopeptin 901 was recorded. This report evaluates the effect of nutrients on the production of biomass and toxins, which may predict the formation and control of blooms of N. spumigena in the Baltic Sea. It also provides information to improve the growth conditions to produce high biomass and toxins under suitable conditions, which may be helpful in the research. The results from the current study will also be helpful to predict about possible blooms of N. spumigena in the Baltic Sea with reference to increase or decrease in nitrate and phosphate concentrations.}, doi = {10.1007/s10811-019-02008-z}, eissn = {1573-5176}, issn = {0921-8971}, issue = {2}, journal = {Journal of Applied Phycology}, note = {INFO COMPLETE (Now published, checked and uplated 18/5/2020 LM, Info via contact 10/1/2020 LM) PERMISSION GRANTED (version = AAM; embargo = 12 months; licence = BY-NC; SHERPA = http://sherpa.ac.uk/romeo/issn/0921-8971/ ) DOCUMENT READY (AAM rec'd from contact 10/1/2020 LM) ADDITIONAL INFORMATION: Contact: Hameed, Shaista; Lawton, Linda; Edwards, Christine}, pages = {937-950}, publicationstatus = {Published}, publisher = {Springer}, url = {https://rgu-repository.worktribe.com/output/824320}, volume = {32}, keyword = {Cyanobacterium, Abiotic factors, Toxins, Biomass, Baltic Sea, Nodularin, Nodulopeptin 901}, year = {2020}, author = {Hameed, Shaista and Lawton, Linda A. and Edwards, Christine} }