Comparative metabologenomics analysis of polar actinomycetes.

Soldatou, Sylvia; Eldj�rn, Gr�mur Hj�rleifsson; Ramsay, Andrew; van der Hooft, Justin J.J.; Hughes, Alison H.; Rogers, Simon; Duncan, Katherine R.

Abstract

Biosynthetic and chemical datasets are the two major pillars for microbial drug discovery in the omics era. Despite the advancement of analysis tools and platforms for multi-strain metabolomics and genomics, linking these information sources remains a considerable bottleneck in strain prioritisation and natural product discovery. In this study, molecular networking of the 100 metabolite extracts derived from applying the OSMAC approach to 25 Polar bacterial strains, showed growth media specificity and potential chemical novelty was suggested. Moreover, the metabolite extracts were screened for antibacterial activity and promising selective bioactivity against drug-persistent pathogens such as Klebsiella pneumoniae and Acinetobacter baumannii was observed. Genome sequencing data were combined with metabolomics experiments in the recently developed computational approach, NPLinker, which was used to link BGC and molecular features to prioritise strains for further investigation based on biosynthetic and chemical information. Herein, we putatively identified the known metabolites ectoine and chrloramphenicol which, through NPLinker, were linked to their associated BGCs. The metabologenomics approach followed in this study can potentially be applied to any large microbial datasets for accelerating the discovery of new (bioactive) specialised metabolites.

Citation

SOLDATOU, S., ELDJÁRN, G.H., RAMSAY, A., VAN DER HOOFT, J.J.J., HUGHES, A.H., ROGERS, S. and DUNCAN, K.R. 2021. Comparative metabologenomics analysis of polar actinomycetes. Marine drugs [online], 19(2), article 103. Available from: https://doi.org/10.3390/md19020103

• Journal Article Type: Article
• Acceptance Date: Feb 8, 2021
• Online Publication Date: Feb 10, 2021
• Publication Date: Feb 28, 2021
• Deposit Date: Mar 25, 2021
• Publicly Available Date: Mar 25, 2021
• Journal: Marine Drugs
• Electronic ISSN: 1660-3397
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 19
• Issue: 2
• Article Number: 103
• DOI: https://doi.org/10.3390/md19020103
• Keywords: Actinobacteria; Marine; Polar; Genomics; Metabolomics; Specialised metabolites
• Public URL: https://rgu-repository.worktribe.com/output/1280370