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Metformin and asarone inhibit HepG2 cell proliferation in a high glucose environment by regulating AMPK and Akt signaling pathway. (2021)
Journal Article
DAS, B.K., KNOTT, R.M. and GADAD, P.C. 2021. Metformin and asarone inhibit HepG2 cell proliferation in a high glucose environment by regulating AMPK and Akt signaling pathway. Future journal of pharmaceutical sciences [online], 7, article 43. Available from: https://doi.org/10.1186/s43094-021-00193-8

Background: Metabolic dysregulation is one of the hallmarks of tumor cell proliferation. Evidence indicates the potential role of the 5′adenosine monophosphate-activated protein kinase (AMPK) and protein kinase B/Akt signaling pathway in regulating c... Read More about Metformin and asarone inhibit HepG2 cell proliferation in a high glucose environment by regulating AMPK and Akt signaling pathway..

Meta-analysis demonstrates Gly482Ser variant of PPARGC1A is associated with components of metabolic syndrome within Asian populations. (2019)
Journal Article
BHATTA, P., BERMANO, G., WILLIAMS, H.C. and KNOTT, R.M. 2020. Meta-analysis demonstrates Gly482Ser variant of PPARGC1A is associated with components of metabolic syndrome within Asian populations. Genomics [online], 112(2), pages 1795-1803. Available from: https://doi.org/10.1016/j.ygeno.2019.10.011

To determine the association of peroxisome proliferator activated receptor gamma coactivator 1 Gly482Ser variant with components of metabolic syndrome. Materials and methods:- A systematic search was carried out using Web of Science, PubMed, EMBASE a... Read More about Meta-analysis demonstrates Gly482Ser variant of PPARGC1A is associated with components of metabolic syndrome within Asian populations..

An investigation into the molecular mechanisms of hyperbaric oxygen using a human dermal microvascular endothelial cell line and a porcine retinal explant model. (2019)
Thesis
IWUAGWU, B.U. 2019. An investigation into the molecular mechanisms of hyperbaric oxygen using a human dermal microvascular endothelial cell line and a porcine retinal explant model. Robert Gordon University [online], PhD thesis. Available from: https://openair.rgu.ac.uk

Diabetes is a debilitating metabolic condition with associated vascular complications that are a major cause of morbidity and mortality. Endothelial dysfunction is central to microvascular complications such as diabetic retinopathy (DR) and impaired... Read More about An investigation into the molecular mechanisms of hyperbaric oxygen using a human dermal microvascular endothelial cell line and a porcine retinal explant model..

Complexation of novel thiomers and insulin to protect against in vitro enzymatic degradation: towards oral insulin delivery. (2018)
Journal Article
IBIE, C.O., KNOTT, R.M. and THOMPSON, C.J. 2019. Complexation of novel thiomers and insulin to protect against in vitro enzymatic degradation: towards oral insulin delivery. Drug development and industrial pharmacy [online], 45(1), pages 67-75. Available from: https://doi.org/10.1080/03639045.2018.1517776

A significant barrier to oral insulin delivery is its enzymatic degradation in the gut. Nano-sized polymer-insulin polyelectrolyte complexes (PECS) have been developed to protect insulin against enzymatic degradation. Poly(allylamine) (Paa) was trime... Read More about Complexation of novel thiomers and insulin to protect against in vitro enzymatic degradation: towards oral insulin delivery..

Trigonelline prevents high cholesterol and high fat diet induced hepatic lipid accumulation and lipo-toxicity in C57BL/6J mice, via restoration of hepatic autophagy. (2018)
Journal Article
SHARMA, L., LONE, N.A., KNOTT, R.M., HASSAN, A., ABDULLAH, T. 2018. Trigonelline prevents high cholesterol and high fat diet induced hepatic lipid accumulation and lipo-toxicity in C57BL/6J mice, via restoration of hepatic autophagy. Food and chemical toxicology [online], 121, pages 283-296. Available from: https://doi.org/10.1016/j.fct.2018.09.011

Non-alcoholic fatty liver disease (NAFLD) is often linked with impaired hepatic autophagy. Here, we studied the alterations in hepatocellular autophagy by high cholesterol and high-fat diet (HC-HF) diet in C57BL/6J mice, and by palmitic acid (PA), in... Read More about Trigonelline prevents high cholesterol and high fat diet induced hepatic lipid accumulation and lipo-toxicity in C57BL/6J mice, via restoration of hepatic autophagy..

An investigation of genetic polymorphism in association with Type 2 diabetes and metabolic syndrome. (2018)
Thesis
BHATTA, P. 2018. An investigation of genetic polymorphism in association with Type 2 diabetes and metabolic syndrome. Robert Gordon University, PhD thesis.

Type 2 diabetes and metabolic syndrome are the metabolic disorders which constitute a major public health problem in both developed and developing countries. Various studies have suggested the genetic susceptibility to the disorders. The main aim of... Read More about An investigation of genetic polymorphism in association with Type 2 diabetes and metabolic syndrome..

Use of a three dimensional porcine retinal explant model to detect HIF1 alpha and targets for understanding diabetic retinopathy. (2017)
Presentation / Conference
IWUAGWU, B.U., CRUICKSHANK, S.F., ROWE, I. and KNOTT, R.M. 2017. Use of a three dimensional porcine retinal explant model to detect HIF1 alpha and targets for understanding diabetic retinopathy. Presented at the American Society for Biochemistry and Molecular Biology annual meeting 2017 (ASBMB'17), 22-26 April 2017, Chicago, USA.

Diabeticretinopathy (DR) is a major microvascular complication associated with diabetes mellitus and is a leading cause of blindness. Hypoxia and hyperglycemia are suggested to play crucial pathophysiological roles, but the exact mechanisms are compl... Read More about Use of a three dimensional porcine retinal explant model to detect HIF1 alpha and targets for understanding diabetic retinopathy..

Preformulation of cysteamine gels for treatment of the ophthalmic complications in cystinosis. (2016)
Journal Article
MCKENZIE, B., KAY, G., MATTHEWS, K.H., KNOTT, R. and CAIRNS, D. 2016. Preformulation of cysteamine gels for treatment of the ophthalmic complications in cystinosis. International journal of pharmaceutics [online], 515(1-2), pages 575-582. Available from: https://doi.org/10.1016/j.ijpharm.2016.10.044

Nephropathic cystinosis is a rare autosomal recessive disease characterised by raised lysosomal levels of cystine in the cells of all organs. It is treated by regular administration of the aminothiol, cysteamine. Corneal crystal deposition is one of... Read More about Preformulation of cysteamine gels for treatment of the ophthalmic complications in cystinosis..

Role of nuclear factor kappa B (NFkB) in the vascular complications of diabetes. (2016)
Thesis
AL ALI, F.M. 2016. Role of nuclear factor kappa B (NFkB) in the vascular complications of diabetes. Robert Gordon University, MRes thesis.

Diabetes mellitus (DM) is the most common metabolic disease worldwide. This condition accounts for the majority of renal failure and blindness in adults aged between 20 to 74 years of age. After 20 years, some of type 1 diabetic patients and around 6... Read More about Role of nuclear factor kappa B (NFkB) in the vascular complications of diabetes..

The hen’s egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: quantification using Photoshop® and ImageJ. (2015)
Journal Article
MCKENZIE, B., KAY, G., MATTHEWS, K.H., KNOTT, R.M. and CAIRNS, D. 2015. The hen’s egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: quantification using Photoshop® and ImageJ. International journal of pharmaceutics [online], 490(1-2), pages 1-8. Available from: https://doi.org/10.1016/j.ijpharm.2015.05.023

A modified hen’s egg chorioallantoic membrane (HET-CAM) test has been developed, combining ImageJ analysis with Adobe® Photoshop®. The irritation potential of an ophthalmic medicine can be quantified using this method, by monitoring damage to blood v... Read More about The hen’s egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: quantification using Photoshop® and ImageJ..

Synthesis, characterisation and in vitro evaluation of novel thiolated derivatives of polyallylamine and quaternised polyallylamine. (2015)
Journal Article
IBIE, C.O., THOMPSON, C.J. and KNOTT, R. 2015. Synthesis, characterisation and in vitro evaluation of novel thiolated derivatives of polyallylamine and quaternised polyallylamine. Colloid and polymer science [online], 293(6), pages 1737-1748. Available from: https://doi.org/10.1007/s00396-015-3561-4

Polyallylamine (Paa) was quaternised by methylation of its primary amines using methyl iodide to yield quaternised Paa (QPaa). Average level of polymer quaternisation was determined by elemental analysis and was found to be 72 ± 2mol%. Subsequent thi... Read More about Synthesis, characterisation and in vitro evaluation of novel thiolated derivatives of polyallylamine and quaternised polyallylamine..

In-vitro evaluation of the effect of polymer structure on uptake of novel polymer-insulin polyelectrolyte complexes by human epithelial cells. (2014)
Journal Article
IBIE, C., KNOTT, R. and THOMPSON, C.J. 2015. In-vitro evaluation of the effect of polymer structure on uptake of novel polymer-insulin polyelectrolyte complexes by human epithelial cells. International journal of pharmaceutics [online], 479(1), pages 103-117. Available from: https://doi.org/10.1016/j.ijpharm.2014.12.058

The biocompatibility and cellular uptake of polymer, insulin polyelectrolyte complexes (PECs) prepared using polyallylamine-based polymers was evaluated in-vitro using Caco-2 cell monolayers as a predictive model for human small intestinal epithelial... Read More about In-vitro evaluation of the effect of polymer structure on uptake of novel polymer-insulin polyelectrolyte complexes by human epithelial cells..

Novel polyelectrolyte complexes for oral insulin delivery. (2013)
Thesis
IBIE, C.O. 2013. Novel polyelectrolyte complexes for oral insulin delivery. Robert Gordon University, PhD thesis.

Oral delivery of insulin used for the management of Type 1 diabetes could be referred to as one of the major long-term goals of diabetes research. However, the bioavailability of orally-administrered insulin is significantly compromised by enzymatic... Read More about Novel polyelectrolyte complexes for oral insulin delivery..

The molecular mechanisms of diabetes mediated impaired wound healing and the development of therapeutic strategies. (2011)
Thesis
GADAD, P.C. 2011. The molecular mechanisms of diabetes mediated impaired wound healing and the development of therapeutic strategies. Robert Gordon University, PhD thesis.

Increased levels of blood glucose are associated with the vascular complications of diabetes. Microvascular complications lead to delayed wound healing in patients suffering from diabetes. Hypoxia and hyperglycaemia characterise a wound environment o... Read More about The molecular mechanisms of diabetes mediated impaired wound healing and the development of therapeutic strategies..

Studies on the role of the endocannabinoid anandamide, as a possible modulator of events during neointimal formation. (2010)
Thesis
SKENE, K.E. 2010. Studies on the role of the endocannabinoid anandamide, as a possible modulator of events during neointimal formation. Robert Gordon University, PhD thesis.

Neointimal formation is a complex process that occurs due to the over compensatory healing response produced by the vessel following injury. Three key events, SMC proliferation, SMC migration and the inflammatory response, occur in unison to drive th... Read More about Studies on the role of the endocannabinoid anandamide, as a possible modulator of events during neointimal formation..

Cytochrome P450 mRNA profile in human breast cancer cell lines. (2008)
Thesis
WARASIHA, B. 2008. Cytochrome P450 mRNA profile in human breast cancer cell lines. Robert Gordon University, PhD thesis.

Cytochrome P450 enzymes (P450s) are involved in cancer development and treatment due to their roles in the oxidative metabolism of various compounds, both endogenous (e.g. oestrogen) and exogenous (e.g. tamoxifen). Intermediate P450 metabolites deriv... Read More about Cytochrome P450 mRNA profile in human breast cancer cell lines..

Investigation of hypoxia and hyperglycaemia mediated DNA damage in human endothelial cells. (2008)
Thesis
WEIDMANN, A.E. 2008. Investigation of hypoxia and hyperglycaemia mediated DNA damage in human endothelial cells. Robert Gordon University, PhD thesis.

Chronic complications of diabetes mellitus are a pathological consequence of unregulated levels of blood glucose leading to disorders of the vascular system. When investigating the vascular complications the changes in the endothelium are of particul... Read More about Investigation of hypoxia and hyperglycaemia mediated DNA damage in human endothelial cells..

Molecular mechanisms of nutrient mediated regulation of hypoxia inducible factor -1a(HIF-1a) in endothelial cells. (2007)
Thesis
HECTOR, E.E. 2007. Molecular mechanisms of nutrient mediated regulation of hypoxia inducible factor -1a(HIF-1a) in endothelial cells. Robert Gordon University, PhD thesis.

Poorly-controlled diabetes mellitus is associated with the development of chronic vascular complications, which cause morbidity and premature mortality. Many studies have highlighted that maintenance of normal blood glucose levels in all people with... Read More about Molecular mechanisms of nutrient mediated regulation of hypoxia inducible factor -1a(HIF-1a) in endothelial cells..