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A novel in vitro model of the small intestinal epithelium in co-culture with "gut-like" dendritic cells. (2023)
Journal Article
JOHNSTON, L.J., BARNINGHAM, L., CAMPBELL, E.L., CEROVIC, V., DUCKWORTH, C.A., LUU, L., WASTLING, J., DERRICOTT, H. and COOMBES, J.L. 2023. A novel in vitro model of the small intestinal epithelium in co-culture with "gut-like" dendritic cells. Discovery immunology [online], 2(1), article number kyad018. Available from: https://doi.org/10.1093/discim/kyad018

Cross-talk between dendritic cells (DCs) and the intestinal epithelium is important in the decision to mount a protective immune response to a pathogen or to regulate potentially damaging responses to food antigens and the microbiota. Failures in thi... Read More about A novel in vitro model of the small intestinal epithelium in co-culture with "gut-like" dendritic cells..

Specific pathway abundances in the neonatal calf faecal microbiome are associated with susceptibility to Cryptosporidium parvum infection: a metagenomic analysis. [Dataset] (2023)
Dataset
HARES, M.F., GRIFFITHS, B.E., JOHNSON, F., NELSON, C., HALDENBY, S., STEWART, C.J., DUNCAN, J.S., OIKONOMOU, G. and COOMBES, J.L. 2023. Specific pathway abundances in the neonatal calf faecal microbiome are associated with susceptibility to Cryptosporidium parvum infection: a metagenomic analysis. [Dataset]. Animal microbiome [online], 5, article number 43. Available from: https://animalmicrobiome.biomedcentral.com/articles/10.1186/s42523-023-00265-5#Sec20

Cryptosporidium parvum is an apicomplexan, protozoan parasite that invades the small intestinal epithelium of neonatal calves. It causes an acute diarrhoeal disease known as cryptosporidiosis, which is characterised by watery diarrhoea, dehydration,... Read More about Specific pathway abundances in the neonatal calf faecal microbiome are associated with susceptibility to Cryptosporidium parvum infection: a metagenomic analysis. [Dataset].

Specific pathway abundances in the neonatal calf faecal microbiome are associated with susceptibility to Cryptosporidium parvum infection: a metagenomic analysis. (2023)
Journal Article
HARES, M.F., GRIFFITHS, B.E., JOHNSON, F., NELSON, C., HALDENBY, S., STEWART, C.J. and DUNCAN, J.S. 2023. Specific pathway abundances in the neonatal calf faecal microbiome are associated with susceptibility to Cryptosporidium parvum infection: a metagenomic analysis. Animal microbiome [online], 5, article number 43. Available from: https://doi.org/10.1186/s42523-023-00265-5

Cryptosporidium parvum is the main cause of calf scour worldwide. With limited therapeutic options and research compared to other Apicomplexa, it is important to understand the parasites' biology and interactions with the host and microbiome in order... Read More about Specific pathway abundances in the neonatal calf faecal microbiome are associated with susceptibility to Cryptosporidium parvum infection: a metagenomic analysis..

Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface. (2022)
Journal Article
ROMERO-RAMIREZ, A., CASAS-SÁNCHEZ, A., AUTHEMAN, D., DUFFY, C.W., BRANDT, C., CLARE, S., HARCOURT, K., ANDRÉ, M.R., DE ALMEIDA CASTILHO NETO, K.J.G., TEIXEIRA, M.M.G., MACHADO, R.Z., COOMBES, J., FLYNN, R.J., WRIGHT, G.J., JACKSON, A.P. 2022. Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface. PLoS neglected tropical diseases [online], 16(9), article number e0010791. Available from: https://doi.org/10.1371/journal.pntd.0010791

Trypanosoma vivax is a unicellular hemoparasite, and a principal cause of animal African trypanosomiasis (AAT), a vector-borne and potentially fatal livestock disease across sub-Saharan Africa. Previously, we identified diverse T. vivax-specific gene... Read More about Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface..

Cleaved CD95L perturbs in vitro macrophages responses to Toxoplasma gondii. (2022)
Journal Article
TIFFNEY, E.A., COOMBES, J.L., LEGEMBRE, P. and FLYNN, R.J. 2022. Cleaved CD95L perturbs in vitro macrophages responses to Toxoplasma gondii. Microbes and infection [online], 24(5), article number 104952. Available from: https://doi.org/10.1016/j.micinf.2022.104952

Toxoplasma gondii infects approximately 1–2 billion people, and manipulation of the macrophage response is critical to host and parasite survival. A cleaved (cl)-CD95L form can promote cellular migration and we have previously shown that cl-CD95L agg... Read More about Cleaved CD95L perturbs in vitro macrophages responses to Toxoplasma gondii..

Non-canonical autophagy functions of ATG16L1 in epithelial cells limit lethal infection by influenza A virus. (2021)
Journal Article
WANG, Y., SHARMA, P., JEFFERSON, M. et al. 2021. Non-canonical autophagy functions of ATG16L1in epithelial cells limit lethal infection by influenzaA virus. EMBO journal [online], 40(6), article e105543. Available from: https://doi.org/10.15252/embj.202010554

Influenza A virus (IAV) and SARS-CoV-2 (COVID-19) cause pandemic infections where cytokine storm syndrome and lung inflammation lead to high mortality. Given the high social and economic cost of respiratory viruses, there is an urgent need to underst... Read More about Non-canonical autophagy functions of ATG16L1 in epithelial cells limit lethal infection by influenza A virus..

Stem cell‐derived enteroid cultures as a tool for dissecting host‐parasite interactions in the small intestinal epithelium. (2020)
Journal Article
HARES, M.F., TIFFNEY, E.A., JOHNSTON, L.J., LUU, L., STEWART, C.J., FLYNN, R.J. and COOMBES, J.L. 2021. Stem cell-derived enteroid cultures as a tool for dissecting host-parasite interactions in the small intestinal epithelium. Parasite immunology [online], 43(2): parasites and tissue micro-environment, article e12765. Available from: https://doi.org/10.1111/pim.12765

Toxoplasma gondii and Cryptosporidium spp. can cause devastating pathological effects in humans and livestock, and in particular to young or immunocompromised individuals. The current treatment plans for these enteric parasites are limited due to lon... Read More about Stem cell‐derived enteroid cultures as a tool for dissecting host‐parasite interactions in the small intestinal epithelium..

Parasitized natural killer cells do not facilitate the spread of toxoplasma gondii to the brain. (2018)
Journal Article
PETIT-JENTREAU, L., GLOVER, C. and COOMBES, J.L. 2018. Parasitized natural killer cells do not facilitate the spread of toxoplasma gondiito the brain. Parasite immunology [online], 40(4), article e12522. Available from: https://doi.org/10.1111/pim.12522

Toxoplasmagondii is a protozoan parasite capable of invading immune cells and co-opting their migratory pathways to disseminate through the host. Natural Killer (NK) cells can be directly invaded by the parasite and this invasion alters NK cell migra... Read More about Parasitized natural killer cells do not facilitate the spread of toxoplasma gondii to the brain..