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Rapid bioassay-guided isolation of antibacterial clerodane type diterpenoid from Dodonaea viscosa (L.) Jaeq.

Khurram, Muhammad; Lawton, Linda A.; Edwards, Christine; Iriti, Marcello; Hameed, Abdul; Khan, Murad A.; Khan, Farman A.; Rahman, Shafiq Ur

Authors

Muhammad Khurram

Marcello Iriti

Abdul Hameed

Murad A. Khan

Farman A. Khan

Shafiq Ur Rahman



Abstract

Plant extracts are complex matrices and, although crude extracts are widely in use, purified compounds are pivotal in drug discovery. This study describes the application of automated preparative-HPLC combined with a rapid off-line bacterial bioassay, using reduction of a tetrazolium salt as an indicator of bacterial metabolism. This approach enabled the identification of fractions from Dodonaea viscosa that were active against Staphylococcus aureus and Escherichia coli, which, ultimately, resulted in the identification of a clerodane type diterpenoid, 6β-hydroxy-15,16-epoxy-5β, 8β, 9β, 10α-cleroda-3, 13(16), 14-trien-18-oic acid, showing bacteriostatic activity (minimum inhibitory concentration (MIC) = 64–128 µg/mL) against test bacteria. To the best of our knowledge, this is the first report on antibacterial activity of this metabolite from D. viscosa.

Citation

KHURRAM, M., LAWTON, L.A., EDWARDS, C., IRITI, M., HAMEED, A., KHAN, M.A., KHAN, F.A. and RAHMAN, S.U. 2015. Rapid bioassay-guided isolation of antibacterial clerodane type diterpenoid from Dodonaea viscosa (L.) Jaeq. International journal of molecular science [online], 16(9), pages 20290-20307. Available from: https://doi.org/10.3390/ijms160920290

Journal Article Type Article
Acceptance Date Aug 17, 2015
Online Publication Date Aug 27, 2015
Publication Date Sep 30, 2015
Deposit Date Feb 19, 2021
Publicly Available Date Sep 12, 2022
Journal International Journal of Molecular Sciences
Print ISSN 1661-6596
Electronic ISSN 1422-0067
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 16
Issue 9
Pages 20290-20307
DOI https://doi.org/10.3390/ijms160920290
Keywords Dodonaea viscosa; Preparative HPLC; XTT assay; Clerodane diterpenoid
Public URL https://rgu-repository.worktribe.com/output/1148074

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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/

Copyright Statement
© 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).




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