Rapid bioassay-guided isolation of antibacterial clerodane type diterpenoid from Dodonaea viscosa (L.) Jaeq.

Khurram, Muhammad; Lawton, Linda A.; Edwards, Christine; Iriti, Marcello; Hameed, Abdul; Khan, Murad A.; Khan, Farman A.; Rahman, Shafiq Ur

doi:10.3390/ijms160920290

Rapid bioassay-guided isolation of antibacterial clerodane type diterpenoid from Dodonaea viscosa (L.) Jaeq.

Khurram, Muhammad; Lawton, Linda A.; Edwards, Christine; Iriti, Marcello; Hameed, Abdul; Khan, Murad A.; Khan, Farman A.; Rahman, Shafiq Ur

Authors

Muhammad Khurram

Professor Linda Lawton l.lawton@rgu.ac.uk
Professor

Professor Christine Edwards c.edwards@rgu.ac.uk
Professor

Marcello Iriti

Abdul Hameed

Murad A. Khan

Farman A. Khan

Shafiq Ur Rahman

Abstract

Plant extracts are complex matrices and, although crude extracts are widely in use, purified compounds are pivotal in drug discovery. This study describes the application of automated preparative-HPLC combined with a rapid off-line bacterial bioassay, using reduction of a tetrazolium salt as an indicator of bacterial metabolism. This approach enabled the identification of fractions from Dodonaea viscosa that were active against Staphylococcus aureus and Escherichia coli, which, ultimately, resulted in the identification of a clerodane type diterpenoid, 6β-hydroxy-15,16-epoxy-5β, 8β, 9β, 10α-cleroda-3, 13(16), 14-trien-18-oic acid, showing bacteriostatic activity (minimum inhibitory concentration (MIC) = 64–128 µg/mL) against test bacteria. To the best of our knowledge, this is the first report on antibacterial activity of this metabolite from D. viscosa.

Citation

KHURRAM, M., LAWTON, L.A., EDWARDS, C., IRITI, M., HAMEED, A., KHAN, M.A., KHAN, F.A. and RAHMAN, S.U. 2015. Rapid bioassay-guided isolation of antibacterial clerodane type diterpenoid from Dodonaea viscosa (L.) Jaeq. International journal of molecular science [online], 16(9), pages 20290-20307. Available from: https://doi.org/10.3390/ijms160920290

Journal Article Type	Article
Acceptance Date	Aug 17, 2015
Online Publication Date	Aug 27, 2015
Publication Date	Sep 30, 2015
Deposit Date	Feb 19, 2021
Publicly Available Date	Sep 12, 2022
Journal	International Journal of Molecular Sciences
Print ISSN	1661-6596
Electronic ISSN	1422-0067
Publisher	MDPI
Peer Reviewed	Peer Reviewed
Volume	16
Issue	9
Pages	20290-20307
DOI	https://doi.org/10.3390/ijms160920290
Keywords	Dodonaea viscosa; Preparative HPLC; XTT assay; Clerodane diterpenoid
Public URL	https://rgu-repository.worktribe.com/output/1148074

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© 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).