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Degradation of multiple peptides by microcystin-degrader Paucibacter toxinivorans (2C20).

Santos, Allan A.; Soldatou, Sylvia; Freitas de Magalhães, Valeria; Azevedo, Sandra M.F.O.; Camacho-Muñoz, Dolores; Lawton, Linda A.; Edwards, Christine

Authors

Allan A. Santos

Sylvia Soldatou

Valeria Freitas de Magalhães

Sandra M.F.O. Azevedo

Dolores Camacho-Muñoz



Abstract

Since conventional drinking water treatments applied in different countries are inefficient at eliminating potentially toxic cyanobacterial peptides, a number of bacteria have been studied as an alternative to biological filters for the removal of microcystins (MCs). Here, we evaluated the degradation of not only MCs variants (-LR/DM-LR/-RR/-LF/-YR), but also non-MCs peptides (anabaenopeptins A/B, aerucyclamides A/D) by Paucibactertoxinivorans over 7 days. We also evaluated the degradation rate of MC-LR in a peptide mix, with all peptides tested, and in the presence of M. aeruginosa crude extract. Furthermore, biodegradation was assessed for non-cyanobacterial peptides with different chemical structures, such as cyclosporin A, (Glu1)-fibrinopeptide-B, leucine-enkephalin, and oxytocin. When cyanopeptides were individually added, P. toxinivorans degraded them (99%) over 7 days, except for MC-LR and -RR, which decreased by about 85 and 90%, respectively. The degradation rate of MC-LR decreased in the peptide mix compared to an individual compound, however, in the presence of the Microcystis extract, it was degraded considerably faster (3 days). It was noted that biodegradation rates decreased in the mix for all MCs while non-MCs peptides were immediately degraded. UPLC–QTOF–MS/MS allowed us to identify two linear biodegradation products for MC-LR and MC-YR, and one for MC-LF. Furthermore, P. toxinivorans demonstrated complete degradation of non-cyanobacterial peptides, with the exception of oxytocin, where around 50% remained after 7 days. Thus, although P. toxinivorans was previously identified as a MC-degrader, it also degrades a wide range of peptides under a range of conditions, which could be optimized as a potential biological tool for water treatment.

Citation

SANTOS, A.A., SOLDATOU, S., FREITAS DE MAGALHÃES, V., AZEVEDO, S.M.F.O., CAMACHO-MUÑOZ, D., LAWTON, L. A. and EDWARDS, C. 2021. Degradation of multiple peptides by microcystin-degrader Paucibacter toxinivorans (2C20). Toxins [online], 13(4): cyanobacterial toxins: their occurrence, detection and removal, article 265. Available from: https://doi.org/10.3390/toxins13040265

Journal Article Type Article
Acceptance Date Apr 4, 2021
Online Publication Date Apr 8, 2021
Publication Date Apr 30, 2021
Deposit Date Apr 8, 2021
Publicly Available Date Apr 8, 2021
Journal Toxins
Electronic ISSN 2072-6651
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 13
Issue 4
Article Number 265
DOI https://doi.org/10.3390/toxins13040265
Keywords Cyanobacteria; CNN; Water treatment; Hydrogen peroxide; Image recognition; Image segmentation; Aerucyclamide; Anabaenopeptin; Bacterial degradation rate; Cyanopeptides; Microcystin; UPLC-MS
Public URL https://rgu-repository.worktribe.com/output/1299697
Related Public URLs https://rgu-repository.worktribe.com/output/1299712

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