An innovative protocol to select the best growth phase for astaxanthin biosynthesis in H. pluvialis.

Radice, Rosa Paola; Fiorentino, Rocco; De Luca, Maria; Limongi, Antonina Rita; Viviano, Emanuele; Bermano, Giovanna; Martelli, Giuseppe

Abstract

H. pluvialis is a green unicellular microalgae and it is the first producer of natural astaxanthin in the world if subjected to stress conditions such as high light, high salinity and nutrient starvation. Astaxanthin is a powerful antioxidant used in many fields, such as aquaculture, pharmaceutical, food supplements and cosmetic. To obtain a large amount of astaxanthin, researcher focused on the optimisation of H. pluvialis growth. H. pluvialis has four different size growth stage (macrozooids, microzooids, palmelloid and “red non-motile astaxanthin accumulated encysted”), and astaxanthin production occur in the last phase. Recent studies shown that non-motile cells can produce more astaxanthin than motile cells if subjected to light stress. For these reasons, the aim of this study is to find a new and innovative methodology to select and recovery H. pluvialis in his last growth phase thanks to an electrophoretic run, and optimize, in this way, astaxanthin production.

Citation

RADICE, R.P., FIORENTINO, R., DE LUCA, M., LIMONGI, A.R., VIVIANO, E., BERMANO, G. and MARTELLI, G. 2021. An innovative protocol to select the best growth phase for astaxanthin biosynthesis in H. pluvialis. Biotechnology reports [online], 31, article e00655. Available from: https://doi.org/10.1016/j.btre.2021.e00655

• Journal Article Type: Article
• Acceptance Date: Jun 17, 2021
• Online Publication Date: Jun 18, 2021
• Publication Date: Sep 30, 2021
• Deposit Date: Jun 25, 2021
• Publicly Available Date: Jun 25, 2021
• Journal: Biotechnology Reports
• Print ISSN: 2215-017X
• Electronic ISSN: 2215-017X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 31
• Article Number: e00655
• DOI: https://doi.org/10.1016/j.btre.2021.e00655
• Keywords: Astaxanthin; Haematococcus pluvialis; Food supplements; Innovative methodology; Microalgae; Growth optimization
• Public URL: https://rgu-repository.worktribe.com/output/1369862