Laboratory analysis of cyanobacterial toxins and bioassays.

Lawton, Linda A.; Metcalf, James S.; �egura, Bojana; Junek, Ralf; Welker, Martin; T�r�kn�, Andrea; Bl�ha, Lud?k

Abstract

Cyanobacterial toxins or cyanotoxins are a diverse group of compounds with differing chemistries; hence, a single analytical method can rarely be used to evaluate all potential compounds. Toxicity bioassays have been adapted to assess the toxicity of cyanobacterial samples. The most common of these assays is the Enzyme-Linked Immunosorbent Assay kit using antibodies raised to specific cyanotoxins. Testing with bioassays is expected to show whether the sample contains toxic substances and how toxic these substances may potentially be. Laboratory staff handling samples potentially containing toxic cyanobacteria and cyanobacterial toxins is potentially exposed to health hazards, and appropriate protective measures need to be implemented. Following a sampling trip, the samples arriving in the laboratory need to be processed further for analysis or storage. Three aspects are important for sample handling and storage: safety, sample processing to ensure stability and traceability.

Citation

LAWTON, L.A., METCALF, J.S., ŽEGURA, B., JUNEK, R., WELKER, M., TÖRÖKNÉ, A. and BLÁHA, L. 2021. Laboratory analysis of cyanobacterial toxins and bioassays. In Chorus, I. and Welker, M. (eds.) Toxic cyanobacteria in water: a guide to their public health consequences, monitoring and management. Second edition. Abingdon: CRC Press [online], chapter 14, pages 745-800. Available from: https://doi.org/10.1201/9781003081449-14

• Online Publication Date: Mar 8, 2021
• Publication Date: Mar 8, 2021
• Deposit Date: Oct 26, 2021
• Publicly Available Date: Oct 28, 2021
• Publisher: CRC Press
• Pages: 745-800
• Edition: Second edition
• Book Title: Toxic cyanobacteria in water: a guide to their public health consequences, monitoring and management
• Chapter Number: 14
• ISBN: 9780367533311
• DOI: https://doi.org/10.1201/9781003081449-14
• Keywords: Cyanobacteria; Cyanotoxins; Toxins; Toxicity; Water pollution; Water contamination; Bioassays
• Public URL: https://rgu-repository.worktribe.com/output/1507368