A study of the effects of oxypertine on monoaminergic pathways in rat brain using a new HPLC/ECD method.

Curle, Peter F.

Abstract

A new HPLC/ECD system for the measurement of the biogenic amines and their major metabolites has been developed. It comprises of a two column system, one for the analysis of the biogenic amines and one for the metabolites. The preparation of brain samples for analysis has been made as simple as possible (homogenisation in perchloric acid, plus centrifugation) so that all the compounds of interest are present in the sample. The biochemical action of oxypertine has been assessed using the above system. Comparing the action of oxypertine against the action of reserpine has shown a difference between the two compounds. Reserpine causes at least 90% reduction in neuronal amine content while oxypertine caused no reduction in neuronal amine content. These results combined with the results of behavoural studies, suggest that oxypertine disrupts the reserpine resistant (non-vesicular) pool of amines. The Kinetics of amine turnover were also investigated. A computer programme for the analysis of catecholamine turnover rates and times was developed. The programme includes the calculations required for the calculation of the SEM's for turnover rate and time. These differ from previous calculations, as the mathematical assumption on which the calculations are based differ. The effect of oxypertine on catecholamine turnover rates and times was investigated using the above mathematical model and also in the analysis of oxypertine effect on the non-vesicular pool of amines.

Citation

CURLE, P.F. 1986. A study of the effects of oxypertine on monoaminergic pathways in rat brain using a new HPLC/ECD method. Robert Gordon's Institute of Technology, PhD thesis. Hosted on OpenAIR [online]. Available from: https://doi.org/10.48526/rgu-wt-1993192

• Thesis Type: Thesis
• Deposit Date: Aug 30, 2024
• Publicly Available Date: Aug 30, 2024
• DOI: https://doi.org/10.48526/rgu-wt-1993192
• Keywords: High-performance liquid chromatography with electrochemical detection (HPLC/ECD); Biogenic amines; Oxypertine; Brain tissue
• Public URL: https://rgu-repository.worktribe.com/output/1993192
• Award Date: Sep 30, 1986