Daphnia magna exudates impact physiological and metabolic changes in microcystis aeruginosa.

Savic, Gorenka Bojadzija; Edwards, Christine; Briand, Enora; Lawton, Linda; Wiegand, Claudia; Bormans, Myriam

Abstract

While the intracellular function of many toxic and bioactive cyanobacterial metabolites is not yet known, microcystins have been suggested to have a protective role in the cyanobacterial metabolism, giving advantage to toxic over nontoxic strains under stress conditions. The zooplankton grazer Daphnia reduce cyanobacterial dominance until a certain density, which may be supported by Daphnia exudates, affecting the cyanobacterial physiological state and metabolites’ production. Therefore, we hypothesized that D. magna spent medium will impact the production of cyanobacterial bioactive metabolites and affect cyanobacterial photosynthetic activity in the nontoxic, but not the toxic strain. Microcystin (MC-LR and des-MC-LR) producing M. aeruginosa PCC7806 and its non-microcystin producing mutant were exposed to spent media of different D. magna densities and culture durations. D. magna spent medium of the highest density (200/L) cultivated for the shortest time (24 h) provoked the strongest effect. D.magna spent medium negatively impacted the photosynthetic activity of M. aeruginosa PCC7806, as well as the dynamics of intracellular and extracellular cyanobacterial metabolites, while its mutant was unaffected. In the presence of Daphnia medium, microcystin does not appear to have a protective role for the strain. On the contrary, extracellular cyanopeptolin A increased in M. aeruginosa PCC7806 although the potential anti-grazing role of this compound would require further studies.

Citation

SAVIC, G.B., EDWARDS, C., BRIAND, E., LAWTON, L., WIEGAND, C. and BORMANS, M. 2019. Daphnia magna exudates impact physiological and metabolic changes in microcystis aeruginosa. Toxins [online], 11(7), article number 421. Available from: https://doi.org/10.3390/toxins11070421

• Journal Article Type: Article
• Acceptance Date: Jul 17, 2019
• Online Publication Date: Jul 19, 2019
• Publication Date: Jul 31, 2019
• Deposit Date: Jul 29, 2019
• Publicly Available Date: Jul 29, 2019
• Journal: Toxins
• Electronic ISSN: 2072-6651
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 7
• Article Number: 421
• DOI: https://doi.org/10.3390/toxins11070421
• Keywords: Cyanobacteria; Secondary metabolites; PCC7806; Toxic; Mutant; Infochemicals
• Public URL: https://rgu-repository.worktribe.com/output/325258
• Contract Date: Jul 29, 2019