Green synthesized quantum dots as electrochemical labels for sensitive detection of hemorrhagic fever virus.

Hosnedlov�, Bo�ena; Sehnal, Karel; T�thov�, Zuzana; Kepinsk�, Marta; Ruttkay-Nedeck�, Branislav; Fernandez, Carlos; Ofomaja, Augustine Enakpodia; Milnerowicz, Halina; Kizek, Ren�

Abstract

Virus-induced haemorrhagic fevers often show a rapid, dramatic course. Infectivity and mortality are very high. Such viruses are known to attack primates as well as other animal species. Fast, sensitive, selective diagnostics can be very beneficial for early anti-epidemic measures right in the outbreak. Quantum dots (QDs) have excellent features that make them useful as detection labels. Their use increases the sensitivity of the analytical assay. The use of biologically active components in QDs synthesis brings new properties. Quantum dots are nanocrystals that due to their high stability, ease of preparation and biocompatibility are suitable for labeling biomolecules. CdTe QDs modified with mercaptosuccinic acid (MSA), reduced glutathione (GSH), and plant extracts were prepared. Green synthesized CdTe QDs showed emission depending on the amount of extract applied. Thus, QDs were prepared in full-color scale from blue to red. The modified biomolecules of QDs are applicable for the labeling of nucleic acids. Their long-term stability, including their application onto paper, was tested. CdTe QDs exhibit very good electrochemical detection with the limit of detection (LOD) in nanomolar concentration.

Citation

HOSNEDLOVÁ, B., SEHNAL, K., TÓTHOVÁ, Z., KEPINSKÁ, M., RUTTKAY-NEDECKÝ, B., FERNANDEZ, C., OFOMAJA, A.E., MILNEROWICZ, H. and KIZEK, R. 2019. Green synthesized quantum dots as electrochemical labels for sensitive detection of hemorrhagic fever virus. In Proceedings of 11th Nanomaterials international conference 2019 (NANOCON 2019): research and applications, 16-18 October 2019, Brno, Czech Republic. Ostrava: Tanger Ltd, pages 416-422. Available from: https://doi.org/10.37904/nanocon.2019.8650

• Presentation Conference Type: Conference Paper (published)
• Conference Name: 11th Nanomaterials international conference 2019 (NANOCON 2019): research and applications
• Start Date: Oct 16, 2019
• End Date: Oct 18, 2019
• Acceptance Date: Oct 1, 2019
• Online Publication Date: Oct 18, 2019
• Publication Date: Apr 1, 2020
• Deposit Date: Dec 17, 2020
• Publicly Available Date: Dec 17, 2020
• Publisher: Tanger Ltd.
• Peer Reviewed: Peer Reviewed
• Pages: 416-422
• Series ISSN: 2694-930X
• ISBN: 9788087294956
• DOI: https://doi.org/10.37904/nanocon.2019.8650
• Keywords: CdTe; Dangerous viral diseases; Electrochemical biosensor; Electrochemistry; Green synthesis; QDs; Sensors; Viral infection
• Public URL: https://rgu-repository.worktribe.com/output/1017234