Bhrigu Kumar Das
Metformin and asarone inhibit HepG2 cell proliferation in a high glucose environment by regulating AMPK and Akt signaling pathway.
Das, Bhrigu Kumar; Knott, Rachel M.; Gadad, Pramod C.
Authors
Rachel M. Knott
Pramod C. Gadad
Abstract
Metabolic dysregulation is one of the hallmarks of tumor cell proliferation. Evidence indicates the potential role of the 5′adenosine monophosphate-activated protein kinase (AMPK) and protein kinase B/Akt signaling pathway in regulating cell proliferation, survival, and apoptosis. The present study explores the effect of metformin HCl and the combination of α- and β-asarone on the proliferation of HepG2 cells in the presence of high glucose levels simulating the diabetic-hepatocellular carcinoma (HCC) condition. The metformin and asarone reduced HepG2 cell viability in a dose-dependent manner and induced morphological changes as indicated by methyl thiazolyl tetrazolium (MTT) assay. The metformin and asarone arrested the cells at the G0/G1 phase, upregulated the expression of AMPK, and downregulated Akt expression in high glucose conditions as identified by the flow cytometry technique. Further, the upregulated AMPK led to a decrease in the expression of phosphoenolpyruvate carboxykinase-2 (PCK-2) and sterol regulatory element-binding protein-1 (SREBP-1). The anti-proliferative effect of metformin and asarone in the diabetic-HCC condition is mediated via AMPK and Akt pathway.
Citation
DAS, B.K., KNOTT, R.M. and GADAD, P.C. 2021. Metformin and asarone inhibit HepG2 cell proliferation in a high glucose environment by regulating AMPK and Akt signaling pathway. Future journal of pharmaceutical sciences [online], 7, article 43. Available from: https://doi.org/10.1186/s43094-021-00193-8
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 21, 2021 |
Online Publication Date | Feb 12, 2021 |
Publication Date | Dec 31, 2021 |
Deposit Date | Mar 5, 2021 |
Publicly Available Date | Mar 5, 2021 |
Journal | Future journal of pharmaceutical sciences |
Print ISSN | 2314-7253 |
Electronic ISSN | 2314-7253 |
Publisher | Springer |
Peer Reviewed | Peer Reviewed |
Volume | 7 |
Article Number | 43 |
DOI | https://doi.org/10.1186/s43094-021-00193-8 |
Keywords | Asarone; Metformin HCI; HepG2 cells; High glucose; AMPF; Akt |
Public URL | https://rgu-repository.worktribe.com/output/1240105 |
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