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Stem cell?derived enteroid cultures as a tool for dissecting host?parasite interactions in the small intestinal epithelium.

Hares, Miriam F.; Tiffney, Ellen?Alana; Johnston, Luke J.; Luu, Lisa; Stewart, Christopher J.; Flynn, Robin J.; Coombes, Janine L.


Miriam F. Hares

Ellen?Alana Tiffney

Luke J. Johnston

Lisa Luu

Christopher J. Stewart

Robin J. Flynn

Janine L. Coombes


Toxoplasma gondii and Cryptosporidium spp. can cause devastating pathological effects in humans and livestock, and in particular to young or immunocompromised individuals. The current treatment plans for these enteric parasites are limited due to long drug courses, severe side effects, or simply a lack of efficacy. The study of the early interactions between the parasites and the site of infection in the small intestinal epithelium has been thwarted by the lack of accessible, physiologically relevant, and species-specific models. Increasingly, 3D stem cell-derived enteroid models are being refined and developed into sophisticated models of infectious disease. In this review we shall illustrate the use of enteroids to spearhead research into enteric parasitic infections, bridging the gap between cell line cultures and in vivo experiments.


HARES, M.F., TIFFNEY, E.A., JOHNSTON, L.J., LUU, L., STEWART, C.J., FLYNN, R.J. and COOMBES, J.L. 2021. Stem cell-derived enteroid cultures as a tool for dissecting host-parasite interactions in the small intestinal epithelium. Parasite immunology [online], 43(2): parasites and tissue micro-environment, article e12765. Available from:

Journal Article Type Article
Acceptance Date Jun 16, 2020
Online Publication Date Aug 5, 2020
Publication Date Feb 28, 2021
Deposit Date May 3, 2022
Publicly Available Date May 3, 2022
Journal Parasite Immunology
Print ISSN 0141-9838
Electronic ISSN 1365-3024
Publisher Wiley Open Access
Peer Reviewed Peer Reviewed
Volume 43
Issue 2
Article Number e12765
Keywords Enteroid; Organoid; Intestinal epithelium; Apicomplexan; Cryptosporidium parvum; Toxoplasma gondii; Neospora caninum
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