Stem cell?derived enteroid cultures as a tool for dissecting host?parasite interactions in the small intestinal epithelium.

Hares, Miriam F.; Tiffney, Ellen?Alana; Johnston, Luke J.; Luu, Lisa; Stewart, Christopher J.; Flynn, Robin J.; Coombes, Janine L.

Abstract

Toxoplasma gondii and Cryptosporidium spp. can cause devastating pathological effects in humans and livestock, and in particular to young or immunocompromised individuals. The current treatment plans for these enteric parasites are limited due to long drug courses, severe side effects, or simply a lack of efficacy. The study of the early interactions between the parasites and the site of infection in the small intestinal epithelium has been thwarted by the lack of accessible, physiologically relevant, and species-specific models. Increasingly, 3D stem cell-derived enteroid models are being refined and developed into sophisticated models of infectious disease. In this review we shall illustrate the use of enteroids to spearhead research into enteric parasitic infections, bridging the gap between cell line cultures and in vivo experiments.

Citation

HARES, M.F., TIFFNEY, E.A., JOHNSTON, L.J., LUU, L., STEWART, C.J., FLYNN, R.J. and COOMBES, J.L. 2021. Stem cell-derived enteroid cultures as a tool for dissecting host-parasite interactions in the small intestinal epithelium. Parasite immunology [online], 43(2): parasites and tissue micro-environment, article e12765. Available from: https://doi.org/10.1111/pim.12765

• Journal Article Type: Article
• Acceptance Date: Jun 16, 2020
• Online Publication Date: Aug 5, 2020
• Publication Date: Feb 28, 2021
• Deposit Date: May 3, 2022
• Publicly Available Date: May 3, 2022
• Journal: Parasite Immunology
• Print ISSN: 0141-9838
• Electronic ISSN: 1365-3024
• Publisher: Wiley Open Access
• Peer Reviewed: Peer Reviewed
• Volume: 43
• Issue: 2
• Article Number: e12765
• DOI: https://doi.org/10.1111/pim.12765
• Keywords: Enteroid; Organoid; Intestinal epithelium; Apicomplexan; Cryptosporidium parvum; Toxoplasma gondii; Neospora caninum
• Public URL: https://rgu-repository.worktribe.com/output/1580700