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Including supramaximal verification reduced uncertainty in VO2peak response rate.

Renwick, John R.M.; Preobrazenski, Nicholas; Giudice, Michael D.; Swinton, Paul A.; Gurd, Brendon J.


John R.M. Renwick

Nicholas Preobrazenski

Michael D. Giudice

Brendon J. Gurd


Many reports describe using a supramaximal verification phase - exercising at a power output higher than the highest power output recorded during an incremental cardiopulmonary test - to validate VO2max. The impact of verification phases on estimating proportion of VO2max response remains an underexplored area of the individual response literature. This analysis investigated the influence of same-day and separate-day verification phases during repeated measurements (incremental tests - INCR1 and INCR2; incremental tests + supramaximal verification phases - INCR1+ and INCR2+) of VO2max on typical error (TE) and the proportion of individuals classified as responders (i.e. the response rate) following four weeks of aerobic exercise training or a no-exercise control period. Incorporation of supramaximal verification consistently reduced the standard deviation of individual response, typical error, and confidence interval widths. However, variances were statistically similar across all groups (p>0.05). Response rates increased when incorporating either one (INCR1 to INCR1+; 24% to 48%, p=0.07) or two (INCR2 to INCR2+; 28% to 48%, p=0.063) supramaximal verification phase(s). However, response rates remained unchanged when either zero-based thresholds or smallest worthwhile difference response thresholds were used (50% and 90% confidence intervals, all p>0.05). In conclusion, supramaximal verification phases reduced random variability in VO2max response. Compared to separate-day testing (INCR2 and INCR2+), incorporation of a same-day verification (INCR1+) reduced CI widths the most. Researchers should consider using a same-day verification phase to reduce uncertainty and better estimate VO2max response rate to aerobic exercise training.


RENWICK, J.R.M., PREOBRAZENSKI, N., GIUDICE, M.D., SWINTON, P.A. and GURD, B.J. 2024. Including supramaximal verification reduced uncertainty in VO2peak response rate. Applied physiology, nutrition and metabolism [online], 49(1), pages 41-51. Available from:

Journal Article Type Article
Acceptance Date Aug 2, 2023
Online Publication Date Oct 3, 2023
Publication Date Jan 31, 2024
Deposit Date Aug 18, 2023
Publicly Available Date Aug 18, 2023
Journal Applied physiology, nutrition and metabolism
Print ISSN 1715-5312
Electronic ISSN 1715-5320
Publisher NRC Research Press
Peer Reviewed Peer Reviewed
Volume 49
Issue 1
Pages 41-51
Keywords Supramaximal verification phase; Response classification; Maximal oxygen uptake; Incremental testing; Responder; Typical error; Individual response to exercise; Response rate
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