The collagen synthesis response to an acute bout of resistance exercise is greater when ingesting 30 g versus 15 g and 0 g hydrolyzed collagen in resistance-trained young men.

Lee, Joonsung; Tang, Jonathan C.Y.; Dutton, John; Dunn, Rachel; Fraser, William D.; Enright, Kevin; Clark, David R.; Stewart, Claire E.; Erskine, Robert M.

Abstract

Resistance exercise (RE) stimulates collagen synthesis in skeletal muscle and tendon but there is limited and equivocal evidence regarding an effect of collagen supplementation and exercise on collagen synthesis. Furthermore, it is not known if a dose-response exists regarding the effect of hydrolyzed collagen (HC) ingestion and RE on collagen synthesis. We aimed to determine the HC dose-response effect on collagen synthesis following high-intensity RE in resistance-trained young men. Using a double-blind, randomized cross-over design, 10 resistance-trained men (age: 26±3 years; height: 1.77±0.04 m; mass: 79.7±7.0 kg) ingested 0g, 15g or 30g HC with 50mg vitamin C 1h prior to performing four sets' barbell back-squat RE at 10-repetition maximum load, after which they rested for six hours. Blood samples were collected throughout each of the three interventions to analyse procollagen type I N-terminal propeptide (PⅠNP) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX) concentration, and the concentration of 18 collagen amino acids. The serum PINP concentration×time area-under-the-curve (AUC) was greater for 30g (267±79 μg∙L-1∙h) than 15g (235±70 μg∙L-1∙h, P=0.039) and 0g HC (219±88 μg∙L-1∙h, P=0.005) but there was no difference between 0g and 15g HC (P=0.675). The AUCs of glycine and proline were greater for 30g than for 15g and 0g HC (P<0.05). Plasma β-CTX concentration decreased from -1h to +6h (P<0.05), with no differences between interventions. The greater PINP AUC suggests 30g HC ingested prior to high-intensity RE augments whole body collagen synthesis more than 15g and 0g HC in resistance-trained young men.

Citation

LEE, J., TANG, J.C.Y., DUTTON, J., DUNN, R., FRASER, W.D., ENRIGHT, K., CLARK, D.R., STEWART, C.E. and ERSKINE, R.M. [2023]. The collagen synthesis response to an acute bout of resistance exercise is greater when ingesting 30 g versus 15 g and 0 g hydrolyzed collagen in resistance-trained young men. Journal of nutrition [online], In Press. Available from: https://doi.org/10.1016/j.tjnut.2023.10.030

• Journal Article Type: Article
• Acceptance Date: Oct 26, 2023
• Online Publication Date: Nov 24, 2023
• Deposit Date: Nov 24, 2023
• Publicly Available Date: Nov 24, 2023
• Journal: Journal of nutrition
• Print ISSN: 0022-3166
• Electronic ISSN: 1541-6100
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1016/j.tjnut.2023.10.030
• Keywords: Strength training; Vitamin C; Connective tissue; Glycine; Proline; Hydroxyproline
• Public URL: https://rgu-repository.worktribe.com/output/2153085
• Additional Information: This article has been published with separate supporting information. This supporting information has been incorporated into a single file on this repository and can be found at the end of the file associated with this output.