Deferiprone, a non-toxic reagent for determination of iron in samples via sequential injection analysis.

Pragourpun, Kraivinee; Sakee, Uthai; Fernandez, Carlos; Kruanetr, Senee

Abstract

We present for the first time the use of deferiprone as a non-toxic complexing agent for the determination of iron by sequential injection analysis in pharmaceuticals and food samples. The method was based on the reaction of Fe(III) and deferiprone in phosphate buffer at pH 7.5 to give a Fe(III)-deferiprone complex, which showed a maximum absorption at 460 nm. Under the optimum conditions, the linearity range for iron determination was found over the range of 0.05-3.0 μg mL-1 with a correlation coefficient (r2) of 0.9993. The limit of detection and limit of quantitation were 0.032 μg mL-1 and 0.055 μg mL-1, respectively. The relative standard deviation (%RSD) of the method was less than 5.0% (n = 11), and the percentage recovery was found in the range of 96.0-104.0%. The proposed method was satisfactorily applied for the determination of Fe(III) in pharmaceuticals, water and food samples with a sampling rate of 60 h-1.

Citation

PRAGOURPUN, K., SAKEE, U., FERNANDEZ, C. and KRUANETR, S. 2015. Deferiprone, a non-toxic reagent for determination of iron in samples via sequential injection analysis. Spectrochimica acta, part A: molecular and biomolecular spectroscopy [online], 142, pages 110-117. Available from: https://doi.org/10.1016/j.saa.2015.01.081

• Journal Article Type: Article
• Acceptance Date: Jan 29, 2015
• Online Publication Date: Feb 9, 2015
• Publication Date: May 5, 2015
• Deposit Date: Sep 15, 2016
• Publicly Available Date: Sep 15, 2016
• Journal: Spectrochimica acta, part A: molecular and biomolecular spectroscopy
• Print ISSN: 1386-1425
• Electronic ISSN: 1873-3557
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 142
• Pages: 110-117
• DOI: https://doi.org/10.1016/j.saa.2015.01.081
• Keywords: Deferiprone; Iron; Sequential injection
• Public URL: http://hdl.handle.net/10059/1716
• Contract Date: Sep 15, 2016