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Development and validation of a liquid chromatography-tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord.

Paniagua-González, Lucía; Jiménez-Morigosa, Cristian; Lendoiro, Elena; Concheiro, Marta; Cruz, Angelines; López-Rivadulla, Manuel; Castro, Ana de

Authors

Lucía Paniagua-González

Cristian Jiménez-Morigosa

Elena Lendoiro

Marta Concheiro

Angelines Cruz

Manuel López-Rivadulla

Ana de Castro

Abstract

Tobacco exposure during pregnancy is associated with obstetric and fetal complications. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine nicotine, cotinine, and hydroxycotinine (OH-cotinine) in placenta (PL) and umbilical cord (UC). Specimens were homogenized in water, followed by solid-phase extraction. Chromatographic separation was performed using an Atlantis{reg} HILIC Silica column. Detection was accomplished in electrospray in positive mode. Method validation included: linearity (5 to 1000 ng/g), accuracy (86.9 to 105.2% of target concentration in PL, and 89.1 to 105.0% in UC), imprecision (6.8 to 11.8% in PL, and 7.6 to 12.2% in UC), limits of detection (2 ng/g for cotinine and OH-cotinine, and 5 ng/g for nicotine) and quantification (5 ng/g), selectivity (no endogenous or exogenous interferences), matrix effect (-34.1 to -84.5% in PL, %CV = 9.1-24.0%; -18.9 to -84.7% in UC, %CV = 10.2-23.9%), extraction efficiency (60.7 to 131.5% in PL, and 64.1 to 134.2% in UC), and stability 72 h in the autosampler (

Journal Article Type Article
Publication Date Aug 31, 2018
Journal Drug testing and analysis
Print ISSN 1942-7603
Electronic ISSN 1942-7611
Publisher Wiley Open Access
Peer Reviewed Peer Reviewed
Volume 10
Issue 8
Pages 1305-1314
Institution Citation PANIAGUA-GONZÁLEZ. L., JIMÉNEZ-MORIGOSA, C., LENDOIRO, E., CONCHEIRO, M., CRUZ, A., LÓPEZ-RIVADULLA, M. and DE CASTRO, A. 2018. Development and validation of a liquid chromatography-tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord. Drug testing and analysis [online], 10(8), pages 1305-1314. Available from: https://doi.org/10.1002/dta.2381
DOI https://doi.org/10.1002/dta.2381
Keywords Nicotine; Placenta; Umbilical cord; Tobacco; LCMS/MS

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