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Development and validation of a liquid chromatography-tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord.

Paniagua-González, Lucía; Jiménez-Morigosa, Cristian; Lendoiro, Elena; Concheiro, Marta; Cruz, Angelines; López-Rivadulla, Manuel; Castro, Ana de

Authors

Lucía Paniagua-González

Cristian Jiménez-Morigosa

Elena Lendoiro

Marta Concheiro

Angelines Cruz

Manuel López-Rivadulla

Ana de Castro



Abstract

Tobacco exposure during pregnancy is associated with obstetric and fetal complications. This study developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to analyse the placenta (PL) and umbilical cord (UC) for nicotine, cotinine and hydroxycotinine (OH-cotinine). Specimens were homogenized in water, followed by solid-phase extraction. Chromatographic separation was performed using an Atlantis HILIC Silica column. Detection was accomplished in electrospray in positive mode. Method validation included: linearity (5 to 1000 ng/g), accuracy (86.9 to 105.2% of target concentration in PL, and 89.1 to 105.0% in UC), imprecision (6.8 to 11.8% in PL, and 7.6 to 12.2% in UC), limits of detection (2 ng/g for cotinine and OH-cotinine, and 5 ng/g for nicotine) and quantification (5 ng/g), selectivity (no endogenous or exogenous interferences), matrix effect (-34.1 to -84.5% in PL, %CV = 9.1-24.0%; -18.9 to -84.7% in UC, %CV = 10.2-23.9%), extraction efficiency (60.7 to 131.5% in PL, and 64.1 to 134.2% in UC), and stability 72 h in the autosampler ( < 11.5% loss in PL, and < 13% loss in UC). The method was applied to 14 PL and UC specimens from tobacco users during pregnancy. Cotinine (6.8-312.2 ng/g in PL; 6.7-342.3 ng/g in UC) was the predominant analyte, followed by OH-cotinine ( < LOQ-80.2 ng/g in PL; < LOQ-80.5 ng/g in UC) and nicotine (5.7-63.7 ng/g in PL; 5.1-63.3 ng/g in UC). In a future study, this method will be applied to more than 150 specimens collected from a wide clinical study to evaluate the usefulness of maternal hair, meconium, placenta and umbilical cord compared to the maternal interview to detect in utero drug exposure.

Journal Article Type Article
Publication Date Aug 31, 2018
Journal Drug testing and analysis
Print ISSN 1942-7603
Electronic ISSN 1942-7611
Publisher Wiley Open Access
Peer Reviewed Peer Reviewed
Volume 10
Issue 8
Pages 1305-1314
Institution Citation PANIAGUA-GONZÁLEZ. L., JIMÉNEZ-MORIGOSA, C., LENDOIRO, E., CONCHEIRO, M., CRUZ, A., LÓPEZ-RIVADULLA, M. and DE CASTRO, A. 2018. Development and validation of a liquid chromatography-tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord. Drug testing and analysis [online], 10(8), pages 1305-1314. Available from: https://doi.org/10.1002/dta.2381
DOI https://doi.org/10.1002/dta.2381
Keywords Nicotine; Placenta; Umbilical cord; Tobacco; LCMS/MS

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