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The electrochemical and statistical evaluation of isolation of mellitin and apamin from honey bee (Apis Mellifera) venom.

Nguyen, Hoai Viet; Heger, Zbynek; Kominkova, Marketa; Michalek, Petr; Gumulec, Jaromir; Guran, Roman; Pridal, Antonin; Fernandez, Carlos; Hynek, David; Adam, Vojtech; Kizek, Rene


Hoai Viet Nguyen

Zbynek Heger

Marketa Kominkova

Petr Michalek

Jaromir Gumulec

Roman Guran

Antonin Pridal

David Hynek

Vojtech Adam

Rene Kizek


We present in this manuscript for the first time the electrochemical and statistical evaluation of FPLC isolation of mellitin and apamin from honey bee (Apis mellifera) venom. Venoms are extremely complex blends of diverse substances that target a myriad of receptors or ion channels. Therefore, toxins, isolated from venomous organisms can be a valuable tool with diverse biological applications. In this study we decided to optimize the purification of honey bee venom by using fast protein liquid chromatography, to obtain biologically active peptide - melittin (2846.46 Da). Due to a presence of other compounds with similar molecular weight (apamin 2027.34 Da), we optimized a differential pulse voltammetry method with adsorptive transfer technique (AdT DPV), utilizing Brdicka supporting electrolyte for measurements. Typical voltammograms - fingerprints for each substance were obtained and numerical projections of voltammograms were employed to propose an artificial neural network. Our suggested neural network can simply predict the content of each peptide in fraction with following performance: 100 % for training and 100 % for testing.


NGUYEN, H. V., HEGER, Z., KOMINKOVA, M., MICHALEK, P., GUMULEC, J., GURAN, R., PRIDAL, A., FERNANDEZ, C., HYNEK, D., ADAM, V. and KIZEK, R. 2015. The electrochemical and statistical evaluation of isolation of mellitin and apamin from honey bee (Apis Mellifera) venom. International journal of electrochemical science [online] , 10(2), pages 1249-1260. Available from:

Journal Article Type Article
Acceptance Date Dec 4, 2014
Online Publication Date Dec 16, 2014
Publication Date Feb 28, 2015
Deposit Date Jan 22, 2015
Publicly Available Date Jan 22, 2015
Journal International Journal of Electrochemical Science
Print ISSN 1452-3981
Electronic ISSN 1452-3981
Publisher Electrochemical Science Group
Peer Reviewed Peer Reviewed
Volume 10
Issue 2
Pages 1249-1260
Keywords Bee venom; Honey bees; Apis mellifera; Toxicology; Differential pulse voltammetry
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