Zinc-modified nanotransporter of anticancer drugs for targeted therapy: biophysical analysis.

Skalickova, Sylvie; Docekalova, Michaela; Stankova, Martina; Uhlirova, Dagmar; Ruttkay-Nedecky, Branislav; Fernandez, Carlos; Kepinska, Marta; Milnerowicz, Halina; Kizek, Rene

Abstract

Modern anticancer therapy aims to increase the effectiveness of tumor treatment. The aim of this work was to propose a new nanotransporter for targeted delivery of anthracycline antibiotics, which is characterized by its bioavailability, increased uptake of the drug from the bloodstream at the site of tumor tissue and as well as low toxicity to non-target tissue. Chitosan nanoparticles have attracted great attention in the field of drug delivery due to their stability, low toxicity and easy preparation. Deacetylated chitosan skeleton is composed of glucosamine units and has a high density of charged amino groups which allow strong electrostatic interactions with biomolecules, transition metals (Zn, Se) and peptides. We obtained the encapsulation effectiveness of chitosan 20%. Electrochemical detection of the bounded Zn2+ ions into the chitosan structure showed shift from -0.99 to -0.93 V. This result proved the formation of a chitosan-zinc complex. The ability of metallothione in to quench the 2,2-diphenyl-1-picrylhydrazylradicalin the presence of 50 {aelig}M doxorubicin was confirmed by the change of relative absorbance in the range of 50 to 60%.

Citation

SKALICKOVA, S., DOCEKALOVA, M., STANKOVA, M., UHLIROVA, D., RUTTKAY-NEDECKY, B., FERNANDEZ, C., KEPINSKA, M., MILNEROWICZ, H. and KIZEK, R. 2019. Zinc-modified nanotransporter of anticancer drugs for targeted therapy: biophysical analysis. Journal of nanoscience and nanotechnology [online], 19(5), pages 2483-2488. Available from: https://doi.org/10.1166/jnn.2019.15870

• Journal Article Type: Article
• Acceptance Date: Oct 4, 2017
• Online Publication Date: Dec 18, 2018
• Publication Date: May 1, 2019
• Deposit Date: May 21, 2018
• Publicly Available Date: Dec 18, 2018
• Journal: Journal of nanoscience and nanotechnology
• Print ISSN: 1533-4880
• Electronic ISSN: 1533-4899
• Publisher: American Scientific Publishers
• Peer Reviewed: Peer Reviewed
• Volume: 19
• Issue: 5
• Pages: 2483-2488
• DOI: https://doi.org/10.1166/jnn.2019.15870
• Keywords: Breast cancer; Chitosan; Doxorubicin; Nanoparticles; Zinc
• Public URL: http://hdl.handle.net/10059/2930
• Contract Date: May 21, 2018