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Using next generation sequencing to determine diversity of horse intestinal worms: identifying the equine 'nemabiome'.

Mitchell, Catriona J.; O'Sullivan, Catherine M.; Pinloche, Eric; Wilkinson, Toby; Morphew, Russell M.; McEwan, Neil R.

Authors

Catriona J. Mitchell

Catherine M. O'Sullivan

Eric Pinloche

Toby Wilkinson

Russell M. Morphew

Neil R. McEwan

Abstract

Next generation sequencing of DNA from nematode eggs has been utilised to give the first account of the equine ‘nemabiome’. In all equine faecal samples investigated, multiple species of Strongylidae were detected; ranging from 7.5 (SEM 0.79) with 99+% identity to sequences in the NCBI database to 13.3 (SEM 0.80) with 90+% identity. This range is typical of the number of species described previously in morphological studies using large quantities of digesta per animal. However, the current method is non-invasive, relies on DNA analysis avoiding the need for specialist microscopy identification and can be carried out with small samples providing significant advantages over current methods.

Journal Article Type Article
Publication Date Mar 31, 2019
Journal Journal of equine science
Electronic ISSN 1347-7501
Publisher New Publisher Required
Peer Reviewed Peer Reviewed
Volume 30
Issue 1
Pages 1-5
Institution Citation MITCHELL, C.J., O'SULLIVAN, C.M., PINLOCHE, E., WILKINSON, T., MORPHEW, R.M. and MCEWAN, N.R. 2019. Using next generation sequencing to determine diversity of horse intestinal worms: identifying the equine 'nemabiome'. Journal of equine science [online], 30(1), pages 1-5. Available from: https://doi.org/10.1294/jes.30.1
DOI https://doi.org/10.1294/jes.30.1
Keywords Horse; Faecal eggs; Nemabiome; Diversity
Additional Information McEwan, Neil -- Panel A

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