Morphological changes in MCF-7 human breast cancer cells in response to Bis-Naphthalimidopropylspermidine-treatment.

Pavlov, V.; Rodilla, V.; Kong Thoo Lin, P.

Abstract

The effects of bis-naphthalimidopropylspermidine (BNIPSpd), a representative of a novel class of polyamine derivatives with antiproliferative properties on the morphology of MCF-7 human breast cancer cells were studied. BNIPSpd was shown to cause cytoplasmic shrinkage, observed by monitoring the cells by means of phase-contrast microscopy. Chromatin condensation and nucleolar disintegration were also detected by Hoechst 33342 and Giemsa staining the cells, treated with 10 μM BNIPSpd for 8h. Taken together the morhological changes observed strongly suggested that the growth inhibition of MCF- 7 cells in response to BNIPSpd-treatment is realized via an induction of apoptosis. Moreover, nucleolus appears to be an important target in the mode of cytotoxic action of this novel polyamine derivative.

Citation

PAVLOV, V., RODILLA, V. and KONG THOO LIN, P. 2002. Morphological changes in MCF-7 human breast cancer cells in response to Bis-Naphthalimidopropylspermidine-treatment. Biotechnology and biotechnological equipment [online], 16(1), pages 118-123. Available from: https://doi.org/10.1080/13102818.2002.10819165

• Journal Article Type: Article
• Online Publication Date: Apr 15, 2014
• Publication Date: Jun 30, 2002
• Deposit Date: Aug 23, 2022
• Publicly Available Date: Mar 27, 2023
• Journal: Biotechnology & Biotechnological Equipment
• Print ISSN: 1310-2818
• Electronic ISSN: 1314-3530
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• Volume: 16
• Issue: 1
• Pages: 118-123
• DOI: https://doi.org/10.1080/13102818.2002.10819165
• Keywords: Bis-naphthalimidopropylspermidine; Human breast cancer cells; Cytoplasmic shrinkage; Phase-contrast microscopy
• Public URL: https://rgu-repository.worktribe.com/output/866109