Pseudonocardia abyssalis sp. nov. and pseudonocardia oceani sp. nov., two novel actinomycetes isolated from the deep Southern Ocean.

Parra, Jonathan; Soldatou, Sylvia; Rooney, Liam M.; Duncan, Katherine R.

Abstract

The actinomycetes strains KRD168T and KRD185T were isolated from sediments collected from the deep Southern Ocean and, in this work, they are described as representing two novel species of the genus Pseudonocardia through a polyphasic approach. Despite sharing >99% 16S rRNA gene sequence similarity with other members of the genus, comparative genomic analysis allowed species delimitation based on average nucleotide identity and digital DNA–DNA hybridization. The KRD168T genome is characterized by a size of 6.31 Mbp and a G+C content of 73.44 mol%, while the KRD185T genome has a size of 6.82 Mbp and a G+C content of 73.98 mol%. Both strains contain meso-diaminopimelic acid as the diagnostic diamino acid, glucose as the major whole-cell sugar, MK-8(H4) as a major menaquinone and iso-branched hexadecanoic acid as a major fatty acid. Biochemical and fatty acid analyses also revealed differences between these strains and their phylogenetic neighbours, supporting their status as distinct species. The names Pseudonocardia abyssalis sp. nov. (type strain KRD168T=DSM 111918T=NCIMB 15270T) and Pseudonocardia oceani (type strain KRD185T=DSM 111919T=NCIMB 15269T) are proposed.

Citation

PARRA, J., SOLDATOU, S., ROONEY, L.M. and DUNCAN, K.R. 2021. Pseudonocardia abyssalis sp. nov. and pseudonocardia oceani sp. nov., two novel actinomycetes isolated from the deep Southern Ocean. International journal of systematic and evolutionary microbiology [online], 71(9), article 005032. Available from: https://doi.org/10.1099/ijsem.0.005032

• Journal Article Type: Article
• Acceptance Date: Sep 28, 2021
• Online Publication Date: Sep 28, 2021
• Publication Date: Sep 30, 2021
• Deposit Date: Jan 11, 2022
• Publicly Available Date: Jan 11, 2022
• Journal: International Journal of Systematic and Evolutionary Microbiology
• Print ISSN: 1466-5026
• Electronic ISSN: 1466-5034
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 71
• Issue: 9
• Article Number: 005032
• DOI: https://doi.org/10.1099/ijsem.0.005032
• Keywords: Actinobacteria; Ocean; Pseudonocardia; Sediments
• Public URL: https://rgu-repository.worktribe.com/output/1569549
• Additional Information: The accompanying file with this output contains supplementary material of four tables and 3 figures and can be found at the end of the main text (pages 10-15).