Samantha L. Sewell
Heterogeneous Fenton's-like catalyst potentiation of hydrogen peroxide disinfection: an investigation into mechanisms of action.
Sewell, Samantha L.; Owen, Lucy; Laird, Katie; Huddersman, Katherine D.; Walsh, Susannah E.
Katherine D. Huddersman
Professor Susie Walsh Susannah.Walsh@rgu.ac.uk
This study aimed to establish the mechanisms of action (MOA) of a novel surface-functionalised polyacrylonitrile (PAN) catalyst, which was previously shown to have potent antimicrobial activity in conjunction with hydrogen peroxide (H2O2). Bactericidal activity was determined using a disinfectant suspension test. The MOA was investigated by measuring loss of 260nm absorbing material, membrane potential and permeability assays, analysis of intra- and extracellular ATP and pH, and tolerance to sodium chloride and bile salts. The catalyst lowered sub-lethal concentrations of H2O2 from 0.2% to 0.09%. H2O2 ± 3g PAN catalyst significantly (p ≤ 0.05) reduced sodium chloride and bile salt tolerance, suggesting the occurance of sublethal cell membrane damage. The catalyst significantly increased (p ≤ 0.05) N-Phenyl-l-napthylamine uptake (1.51-fold) and leakage of nucleic acids, demonstrating increased membrane permeability. A significant (p ≤ 0.05) loss of membrane potential (0.015 a.u.), coupled with pertubation of intracellular pH homeostasis and depletion of intracellular ATP, suggests potentiation of H2O2-mediated cell membrane damage. This is the first study to investigate the catalyst's antimicrobial mechanism of action, with the cytoplasmic membrane being a target for cellular injury. Understanding the MOA by which the catalyst potentiates H2O2 could lead to optimisation of concentrations and contact times needed for disinfection in a wide range of settings.
SEWELL, S.L., OWEN, L., LAIRD, K., HUDDERSMAN, K.D. and WALSH, S.E. . Heterogeneous Fenton's-like catalyst potentiation of hydrogen peroxide disinfection: an investigation into mechanisms of action. Journal of applied microbiology [online], (accepted).
|Journal Article Type||Article|
|Acceptance Date||Oct 18, 2022|
|Deposit Date||Dec 19, 2022|
|Journal||Journal of applied microbiology|
|Peer Reviewed||Peer Reviewed|
|Keywords||Mechanism of action; Hydrogen peroxide; Fenton; Disinfection; Polyacrylonitrile|
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