Skip to main content

Research Repository

Advanced Search

Heterogeneous Fenton's-like catalyst potentiation of hydrogen peroxide disinfection: an investigation into mechanisms of action.

Sewell, Samantha L.; Owen, Lucy; Laird, Katie; Huddersman, Katherine D.; Walsh, Susannah E.

Authors

Samantha L. Sewell

Lucy Owen

Katie Laird

Katherine D. Huddersman



Abstract

This study aimed to establish the mechanisms of action (MOA) of a novel surface-functionalised polyacrylonitrile (PAN) catalyst, which was previously shown to have potent antimicrobial activity in conjunction with hydrogen peroxide (H2O2). Bactericidal activity was determined using a disinfectant suspension test. The MOA was investigated by measuring loss of 260nm absorbing material, membrane potential and permeability assays, analysis of intra- and extracellular ATP and pH, and tolerance to sodium chloride and bile salts. The catalyst lowered sub-lethal concentrations of H2O2 from 0.2% to 0.09%. H2O2 ± 3g PAN catalyst significantly (p ≤ 0.05) reduced sodium chloride and bile salt tolerance, suggesting the occurance of sublethal cell membrane damage. The catalyst significantly increased (p ≤ 0.05) N-Phenyl-l-napthylamine uptake (1.51-fold) and leakage of nucleic acids, demonstrating increased membrane permeability. A significant (p ≤ 0.05) loss of membrane potential (0.015 a.u.), coupled with pertubation of intracellular pH homeostasis and depletion of intracellular ATP, suggests potentiation of H2O2-mediated cell membrane damage. This is the first study to investigate the catalyst's antimicrobial mechanism of action, with the cytoplasmic membrane being a target for cellular injury. Understanding the MOA by which the catalyst potentiates H2O2 could lead to optimisation of concentrations and contact times needed for disinfection in a wide range of settings.

Citation

SEWELL, S.L., OWEN, L., LAIRD, K., HUDDERSMAN, K.D. and WALSH, S.E. [2022]. Heterogeneous Fenton's-like catalyst potentiation of hydrogen peroxide disinfection: an investigation into mechanisms of action. Journal of applied microbiology [online], (accepted).

Journal Article Type Article
Acceptance Date Oct 18, 2022
Deposit Date Dec 19, 2022
Journal Journal of applied microbiology
Print ISSN 1364-5072
Electronic ISSN 1365-2672
Publisher Wiley
Peer Reviewed Peer Reviewed
Keywords Mechanism of action; Hydrogen peroxide; Fenton; Disinfection; Polyacrylonitrile
Public URL https://rgu-repository.worktribe.com/output/1840447

This file is under embargo due to copyright reasons.

Contact publications@rgu.ac.uk to request a copy for personal use.






You might also like



Downloadable Citations