Quantitative analysis of the Ah receptor signalling pathway.

McFadyen, Morag C.E.; Rooney, Patrick H.; Murray, Graeme I.

Abstract

The aryl hydrocarbon receptor (AhR) is a ligand activated receptor. On dimerisation with the aryl hydrocarbon nuclear translocator (ARNT), AhR binds to xenobiotic responsive elements (XREs) that promote the activation of a battery of genes, including the cytochrome P450 drug metabolising enzymes CYP1A1 and CYP1B1. These two P450s are known to demonstrate distinct cell type expression. The aim of this study was to investigate the mechanism of cellular regulation of CYP1A1 and CYP1B1 by real-time quantitative RT-PCR in three cell lines known to differentially express CYP1A1 and CYP1B1 mRNA (MCF7, HEPG2 and MOG-G-CCM).

Citation

MCFADYEN, M.C.E., ROONEY, P.H. and MURRAY, G.I. 2002. Quantitative analysis of the Ah receptor signalling pathway. Presented at the 2002 British cancer research meeting (BCRM 2002), 30 June - 3 July 2002, Glasgow, UK.

• Presentation Conference Type: Poster
• Conference Name: 2002 British cancer research meeting (BCRM 2002)
• Conference Location: Glasgow, UK
• Start Date: Jun 30, 2002
• End Date: Jul 3, 2002
• Deposit Date: Mar 10, 2023
• Publicly Available Date: Mar 10, 2023
• Publisher: Springer
• Keywords: Cytochrome P450 enzymes; Cellular regulation mechanisms; Aryl hydrocarbon receptor
• Public URL: https://rgu-repository.worktribe.com/output/1905826
• Additional Information: The extended abstract for this poster has been published with the following citation: MCFADYEN, M.C.E., ROONEY, P.H. and MURRAY, G.I. 2002. Quantitative analysis of the Ah receptor signalling pathway. British journal of cancer [online], 86(Suppl 1): abstracts from the 2002 British cancer research meeting (BCRM 2002), 30 June - 3 July 2002, Glasgow, UK, page S84, poster number P165. Available from: https://doi.org/10.1038/sj.bjc.6600378