Assessment of a putative oncogene ZNF217 within amplicon 20q by multiplex quantitative real-time PCR.

Rooney, P.H.; Boonsong, A.; McFadyen, M.; McLeod, H.L.; Cassidy, J.; Murray, G.I.

Abstract

Detection of gene amplification is a recognised process through which oncogenes can be identified. In this study, we have coupled two powerful techniques - laser capture-microdissection and multiplex quantitative real-time PCR - to quickly and accurately assess the gene copy number of candidate oncogene ZNF217 in colon cancer. We found that ZNF217 amplification is a frequent event in colon cancer (48/81 tumours = 59.3%) and that the extent of its amplification varies markedly between tumours (range 3 - 13 copies).

Citation

ROONEY, P., BOONSONG, A., MCFADYEN, M., MCLEOD, H.L., CASSIDY, J. and MURRAY, G.I. 2002. Assessment of a putative oncogene ZNF217 within amplicon 20q by multiplex quantitative real-time PCR. Presented at the 2002 British cancer research meeting (BCRM 2002), 30 June - 3 July 2002, Glasgow, UK.

• Presentation Conference Type: Lecture
• Conference Name: 2002 British cancer research meeting (BCRM 2002)
• Conference Location: Glasgow, UK
• Start Date: Jun 30, 2002
• End Date: Jul 3, 2002
• Deposit Date: Mar 10, 2023
• Publicly Available Date: Mar 10, 2023
• Keywords: Gene amplification; Oncogene identification; Oncogene ZNF217; Colon cancer
• Public URL: https://rgu-repository.worktribe.com/output/1905835
• Additional Information: The extended abstract for this presentation has been published with the following citation: ROONEY, P., BOONSONG, A., MCFADYEN, M., MCLEOD, H.L., CASSIDY, J. and MURRAY, G.I. 2002. Assessment of a putative oncogene ZNF217 within amplicon 20q by multiplex quantitative real-time PCR. British journal of cancer [online], 86(Suppl 1): abstracts from the 2002 British cancer research meeting (BCRM 2002), 30 June - 3 July 2002, Glasgow, UK, page S32, abstract number 8.5. Available from: https://doi.org/10.1038/sj.bjc.6600406