Microbubble-liposome conjugate: payload evaluation of potential theranostic vehicle.

Malik, Ritu; Pancholi, Ketan; Melzer, Andreas

Abstract

Liposome–microbubble conjugates are considered as better targeted drug delivery vehicles compared to microbubbles alone. The microbubble in the integrated drug delivery system delivers the drug intracellularly on the target, whereas the liposome component allows loading of high drug dose and extravasation through leaky vasculature. In this work, a new high yielding microbubble production method was used to prepare microbubbles for formulation of the liposome-conjugated drug delivery system. In formulation process, the prepared liposome of 200 nm diameter was attached to the microbubble surface using the avidin–biotin interaction. The analysis of the confocal scanning laser microscope images showed that approximately 8 × 108 microbubbles per millilitre (range: 2–7 μm, mean size 5 ± 0.5 μm) can be efficiently conjugated to the liposomes. The method of conjugation was found to be effective in attaching liposome to microbubbles.

Citation

MALIK, R., PANCHOLI, K. and MELZER, A. 2016. Microbubble-liposome conjugate: payload evaluation of potential theranostic vehicle. Nanobiomedicine [online], 3, pages 1-8. Available from: https://doi.org/10.1177/1849543516670806

• Journal Article Type: Article
• Acceptance Date: Sep 1, 2016
• Online Publication Date: Oct 5, 2016
• Publication Date: Dec 31, 2016
• Deposit Date: Jan 5, 2017
• Publicly Available Date: Jan 5, 2017
• Journal: Nanobiomedicine
• Electronic ISSN: 1849-5435
• Publisher: SAGE Publications
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Pages: 1-8
• DOI: https://doi.org/10.1177/1849543516670806
• Keywords: Liposome microbubble conjugate; Confocal scanning laser microscopy (CSLM); Image correlation spectroscopy (ICS)
• Public URL: http://hdl.handle.net/10059/2069