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Dual-laboratory validation of a method for the determination of fructans in infant formula and adult nutritionals.

Brunt, Kommer; Sanders, Peter; Spichtig, Véronique; Ernste-Nota, Veronica; Sawicka, Paulina; Iwanoff, Kimberley; Van Soest, Jeroen; Lin, Paul Kong Thoo; Austin, Sean


Kommer Brunt

Peter Sanders

Véronique Spichtig

Veronica Ernste-Nota

Paulina Sawicka

Kimberley Iwanoff

Jeroen Van Soest

Paul Kong Thoo Lin

Sean Austin


Until recently, only two AOAC Official Methods SM have been available for the analysis of fructans: Method 997.08 and Method 999.03. Both are based on the analysis of the fructan component monosaccharides (glucose and fructose) after hydrolysis. The two methods have some limitations due to the strategies used for removing background interferences (such as from sucrose, {esc}ga{esc}s-glucooligosaccharides, and free sugars). The method described in this paper has been developed to overcome those limitations. The method is largely based on Method 999.03 and uses combined enzymatic and SPE steps to remove the interfering components without impacting the final analytical result. The method has been validated in two laboratories on infant formula and adult nutritionals. Recoveries were in the range of 86-119%, with most being in the range of 91-104%. RSDr values were in the range of 0.7-2.6%, with one exception when the fructan concentration was close to the LOQ, resulting in an RSDr of 8.9%. The performance is generally within the requirements outlined in the AOAC Standard Method Performance Requirements (SMPR{reg} 2014.002), which specifies recoveries in the range of 90-110% and RSDr values below 6%.

Journal Article Type Article
Publication Date Jun 30, 2017
Journal Journal of AOAC international
Print ISSN 1060-3271
Electronic ISSN 1944-7922
Publisher AOAC International
Peer Reviewed Peer Reviewed
Volume 100
Issue 3
Pages 753-767
Institution Citation BRUNT, K., SANDERS, P., SPICHTIG, V., ERNSTE-NOTA, V., SAWICKA, P., IWANOFF, K., VAN SOEST, J., KONG THOO LIN, P. and SEAN, A. 2017. Dual-laboratory validation of a method for the determination of fructans in infant formula and adult nutritionals. Journal of AOAC international [online], 100(3), pages 753-767. Available from:
Keywords Fructan; Interfering components; Food products


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