Dual-laboratory validation of a method for the determination of fructans in infant formula and adult nutritionals.

Brunt, Kommer; Sanders, Peter; Ernste-Nota, Veronica; Sawicka, Paulina; Iwanoff, Kimberley; Van Soest, Jeroen; Kong Thoo Lin, Paul; Austin, Sean

doi:10.5740/jaoacint.17-0007

Authors

Kommer Brunt

Peter Sanders

Spichtig

Veronica Ernste-Nota

Paulina Sawicka

Kimberley Iwanoff

Jeroen Van Soest

Professor Paul Kong Thoo Lin p.v.s.kong-thoo-lin@rgu.ac.uk
Professor

Sean Austin

Abstract

Until recently, only two AOAC Official MethodsSM have been available for the analysis of fructans: Method 997.08 and Method 999.03. Both are based on the analysis of the fructan component monosaccharides (glucose and fructose) after hydrolysis. The two methods have some limitations due to the strategies used for removing background interferences (such as from sucrose, α-glucooligosaccharides, and free sugars). The method described in this paper has been developed to overcome those limitations. The method is largely based on Method 999.03 and uses combined enzymatic and SPE steps to remove the interfering components without impacting the final analytical result. The method has been validated in two laboratories on infant formula and adult nutritionals. Recoveries were in the range of 86-119%, with most being in the range of 91-104%. RSDr values were in the range of 0.7-2.6%, with one exception when the fructan concentration was close to the LOQ, resulting in an RSDr of 8.9%. The performance is generally within the requirements outlined in the AOAC Standard Method Performance Requirements (SMPR® 2014.002), which specifies recoveries in the range of 90-110% and RSDr values below 6%.

Citation

BRUNT, K., SANDERS, P., SPICHTIG, V., ERNSTE-NOTA, V., SAWICKA, P., IWANOFF, K., VAN SOEST, J., KONG THOO LIN, P. and SEAN, A. 2017. Dual-laboratory validation of a method for the determination of fructans in infant formula and adult nutritionals. Journal of AOAC international [online], 100(3), pages 753-767. Available from: https://doi.org/10.5740/jaoacint.17-0007

Journal Article Type	Article
Acceptance Date	May 1, 2017
Online Publication Date	May 1, 2017
Publication Date	Jun 30, 2017
Deposit Date	Jun 6, 2017
Publicly Available Date	Jun 6, 2017
Journal	Journal of AOAC international
Print ISSN	1060-3271
Electronic ISSN	1944-7922
Publisher	AOAC International
Peer Reviewed	Peer Reviewed
Volume	100
Issue	3
Pages	753-767
DOI	https://doi.org/10.5740/jaoacint.17-0007
Keywords	Fructan; Interfering components; Food products
Public URL	http://hdl.handle.net/10059/2364