Nasopharyngeal metagenomic deep sequencing data, Lancaster, UK, 2014-2015.

Atkinson, Kate V.; Bishop, Lisa A.; Rhodes, Glenn; Salez, Nicolas; McEwan, Neil R.; Hegarty, Matthew J.; Robey, Julie; Harding, Nicola; Wetherell, Simon; Lauder, Robert M.; Pickup, Roger W.; Wilkinson, Mark; Gatherer, Derek

Abstract

Nasopharyngeal swabs were taken from volunteers attending a general medical practice and a general hospital in Lancaster, UK, and at Lancaster University, in the winter of 2014-2015. 51 swabs were selected based on high RNA yield and allocated to deep sequencing pools as follows: patients with chronic obstructive pulmonary disease; asthmatics; adults with no respiratory symptoms; adults with feverish respiratory symptoms; adults with respiratory symptoms and presence of antibodies against influenza C; paediatric patients with respiratory symptoms (2 pools); adults with influenza C infection (2 pools), giving a total of 9 pools. Illumina sequencing was performed, with data yields per pool in the range of 345.6 megabases to 14 gigabases after removal of reads aligning to the human genome. The data were deposited in the Sequence Read Archive at NCBI, and constitute a resource for study of the viral, bacterial and fungal metagenome of the human nasopharynx in healthy and diseased states and comparison with other metagenomic studies on the human respiratory tract.

Citation

ATKINSON, K.V., BISHOP, L.A., RHODES, G., SALEZ, N., MCEWAN, N.R., HEGARTY, M.J., ROBEY, J., HARDING, N., WETHERELL, S., LAUDER, R.M., PICKUP, R.W., WILKINSON, M. and GATHERER, D. 2017. Nasopharyngeal metagenomic deep sequencing data, Lancaster, UK, 2014-2015. Scientific data [online], 4, article No. 170161. Available from: https://doi.org/10.1038/sdata.2017.161

• Journal Article Type: Article
• Acceptance Date: Sep 6, 2017
• Online Publication Date: Oct 24, 2017
• Publication Date: Nov 30, 2017
• Deposit Date: Nov 16, 2017
• Publicly Available Date: Nov 16, 2017
• Journal: Scientific data
• Print ISSN: 2052-4463
• Electronic ISSN: 2052-4463
• Publisher: Springer
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Article Number: 170161
• DOI: https://doi.org/10.1038/sdata.2017.161
• Keywords: Clinical microbiology; Infectious diseases; Metagenomics; RNA sequencing; Virology
• Public URL: http://hdl.handle.net/10059/2591