Kate V. Atkinson
Nasopharyngeal metagenomic deep sequencing data, Lancaster, UK, 2014-2015.
Atkinson, Kate V.; Bishop, Lisa A.; Rhodes, Glenn; Salez, Nicolas; McEwan, Neil R.; Hegarty, Matthew J.; Robey, Julie; Harding, Nicola; Wetherell, Simon; Lauder, Robert M.; Pickup, Roger W.; Wilkinson, Mark; Gatherer, Derek
Lisa A. Bishop
Neil R. McEwan
Matthew J. Hegarty
Robert M. Lauder
Roger W. Pickup
Nasopharyngeal swabs were taken from volunteers attending a general medical practice and a general hospital in Lancaster, UK, and at Lancaster University, in the winter of 2014-2015. 51 swabs were selected based on high RNA yield and allocated to deep sequencing pools as follows: patients with chronic obstructive pulmonary disease; asthmatics; adults with no respiratory symptoms; adults with feverish respiratory symptoms; adults with respiratory symptoms and presence of antibodies against influenza C; paediatric patients with respiratory symptoms (2 pools); adults with influenza C infection (2 pools), giving a total of 9 pools. Illumina sequencing was performed, with data yields per pool in the range of 345.6 megabases to 14 gigabases after removal of reads aligning to the human genome. The data were deposited in the Sequence Read Archive at NCBI, and constitute a resource for study of the viral, bacterial and fungal metagenome of the human nasopharynx in healthy and diseased states and comparison with other metagenomic studies on the human respiratory tract.
|Journal Article Type||Article|
|Publication Date||Nov 30, 2017|
|Publisher||Springer Nature [academic journals on nature.com]|
|Peer Reviewed||Peer Reviewed|
|Institution Citation||ATKINSON, K.V., BISHOP, L.A., RHODES, G., SALEZ, N., MCEWAN, N.R., HEGARTY, M.J., ROBEY, J., HARDING, N., WETHERELL, S., LAUDER, R.M., PICKUP, R.W., WILKINSON, M. and GATHERER, D. 2017. Nasopharyngeal metagenomic deep sequencing data, Lancaster, UK, 2014-2015. Scientific data [online], 4, article No. 170161. Available from: https://doi.org/10.1038/sdata.2017.161|
|Keywords||Clinical microbiology; Infectious diseases; Metagenomics; RNA sequencing; Virology|
ATKINSON 2017 Nasopharyngeal metagenomic deep