Glassy carbon electrode modified with 7,7,8,8-tetracyanoquinodimethane and graphene oxide triggered a synergistic effect: low-potential amperometric detection of reduced glutathione.

Yuan, Baiqing; Xu, Chunying; Zhang, Renchun; Lv, Donghui; Li, Sujuan; Zhang, Daojun; Liu, Lin; Fernandez, Carlos

Abstract

A sensitive electrochemical sensor based on the synergistic effect of 7,7,8,8-tetracyanoquinodimethane (TCNQ) and graphene oxide (GO) for low-potential amperometric detection of reduced glutathione (GSH) in pH 7.2 phosphate buffer solution (PBS) has been reported. This is the first time that the combination of GO and TCNQ have been successfully employed to construct an electrochemical sensor for the detection of glutathione. The surface of the glassy carbon electrode (GCE) was modified by a drop casting using TCNQ and GO. Cyclic voltammetric measurements showed that TCNQ and GO triggered a synergistic effect and exhibited an unexpected electrocatalytic activity towards GSH oxidation, compared to GCE modified with only GO, TCNQ or TCNQ/electrochemically reduced GO. Three oxidation waves for GSH were found at −0.05, 0.1 and 0.5 V, respectively. Amperometric techniques were employed to detect GSH sensitively using a GCE modified with TCNQ/GO at −0.05 V. The electrochemical sensor showed a wide linear range from 0.25 to 124.3 μM and 124.3 μM to 1.67 mM with a limit of detection of 0.15 μM. The electroanalytical sensor was successfully applied towards the detection of GSH in an eye drop solution.

Citation

YUAN, B., XU, C., ZHANG, R., LV, D., LI, S., ZHANG, D., LIU, L. and FERNANDEZ, C. 2017. Glassy carbon electrode modified with 7,7,8,8-tetracyanoquinodimethane and graphene oxide triggered a synergistic effect: low-potential amperometric detection of reduced glutathione. Biosensors and bioelectronics [online], 96, pages 1-7. Available from: https://doi.org/10.1016/j.bios.2017.04.026

• Journal Article Type: Article
• Acceptance Date: Apr 20, 2017
• Online Publication Date: Apr 21, 2017
• Publication Date: Oct 15, 2017
• Deposit Date: Feb 22, 2018
• Publicly Available Date: Apr 22, 2018
• Journal: Biosensors and Bioelectronics
• Print ISSN: 0956-5663
• Electronic ISSN: 1873-4235
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 96
• Pages: 1-7
• DOI: https://doi.org/10.1016/j.bios.2017.04.026
• Keywords: Electrochemical sensor; 7,7,8,8-tetracyanoquinodimethane (TCNQ); Graphene oxide; Reduced glutathione
• Public URL: http://hdl.handle.net/10059/2777