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Evaluation of the microbiome of decaying alder nodules by next generation sequencing.

McEwan, Neil R.; Wilkinson, Toby; Girdwood, Susan E.; Snelling, Tim J.; Collins, Tatiana; Dougal, Kirsty; Jones, David L.; Godbold, Douglas L.


Neil R. McEwan

Toby Wilkinson

Susan E. Girdwood

Tim J. Snelling

Tatiana Collins

Kirsty Dougal

David L. Jones

Douglas L. Godbold


This work investigated the microbial content of decaying nodules from alders. The 16S rDNA composition of the microbiome of six senescent alder nodules was investigated by 454 sequencing. All nodules still had some Frankia sequences present, but in each case it was only detected at minor levels, with other organisms predominating. Although organisms from three different phyla (Bacteroidetes, Proteobacteria and Actinobacteria) constituted almost all (98% or more) of all sequences, Bacteroidetes were most abundant in four nodules with Proteobacteria being most abundant in the other two. In addition a few families were represented at a level of 10% or more of the total sequences: Sphingobacteriaceae (all 6 nodules); Chitinophagaceae (5 of 6); non-Frankia Actinomycetales (2 of 6); Caulobacteraceae (2 of 6); Flavobacteriaceae (2 of 6); Oxalobacteraceae (1 of 6); and Xanthomoadaceae (1 of 6). Analysis at the genus level showed a diverse range of organisms, with members of the genus Pedobacter being found at an abundant level within most nodules.


MCEWAN, N.R., WILKINSON T., GIRDWOOD, S.E., SNELLING, T.J., COLLINS, T., DOUGAL, K., JONES, D.L. and GODBOLD, D.L. 2017. Evaluation of the microbiome of decaying alder nodules by next generation sequencing. Endocytobiosis and cell research [online], 28(1), pages 14-19. Available from:

Journal Article Type Article
Acceptance Date May 18, 2017
Online Publication Date Jun 2, 2017
Publication Date Dec 31, 2017
Deposit Date Jun 5, 2018
Publicly Available Date Jun 5, 2018
Journal Endocytobiosis and cell research
Print ISSN 0256-1514
Electronic ISSN 1613-8872
Publisher International Society of Endocytobiology
Peer Reviewed Peer Reviewed
Volume 28
Issue 1
Pages 14-19
Keywords Alnus glutinosa; Microbiome; Frankia; Senescent nodules; 454 sequencing
Public URL
Publisher URL


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