Sequence variation in multidrug-resistant plasmid pLUH01, isolated from human nasopharyngeal swabs.

Atkinson, Kate V.; Bishop, Lisa A.; Rhodes, Glenn; Salez, Nicolas; McEwan, Neil R.; Hegarty, Matthew J.; Robey, Julie; Harding, Nicola; Wetherell, Simon; Lauder, Robert M.; Pickup, Roger W.; Wilkinson, Mark; Gatherer, Derek

Abstract

Three variants of the multidrug-resistant plasmid pLUH01 were assembled by deep sequencing from nasopharyngeal swabs. All have a 21-bp deletion in the RS14515 hypothetical gene. Variants 1 through 3 have 2, 6, and 3 nucleotide substitutions, respectively, compared to the pLUH01 reference genome. We named the new plasmid variants pLUH01/Lancaster/2015/1 to pLUH01/Lancaster/2015/3.

Citation

ATKINSON, K.V., BISHOP, L.A., RHODES, G., SALEZ, N., MCEWAN, N.R., HEGARTY, M.J., ROBEY, J., HARDING, N., WETHERELL, S., LAUDER, R.M., PICKUP, R.W., WILKINSON, M. and GATHERER, D. 2018. Sequence variation in multidrug-resistant plasmid pLUH01, isolated from human nasopharyngeal swabs. Microbiology resource announcements [online], 7(1), article number 00835-18. Available from: https://doi.org/10.1128/MRA.00835-18

• Journal Article Type: Article
• Acceptance Date: Jun 18, 2018
• Online Publication Date: Jul 12, 2018
• Publication Date: Jul 31, 2018
• Deposit Date: Jul 24, 2018
• Publicly Available Date: Jul 24, 2018
• Journal: Microbiology resource announcements
• Electronic ISSN: 2576-098X
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 1
• Article Number: 00835-18
• DOI: https://doi.org/10.1128/MRA.00835-18
• Keywords: Variants; Multi drug resistant plasmid pLUH01; Deep sequencing; Lancaster
• Public URL: http://hdl.handle.net/10059/3014
• Contract Date: Jul 24, 2018