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Effect of Entodinium caudatum on starch intake and glycogen formation by Eudiplodinium maggii in the rumen and reticulum.

Bełzecki, Grzegorz; McEwan, Neil R.; Kowalik, Barbara; Michałowski, Tadeusz; Miltko, Renata

Authors

Grzegorz Bełzecki

Neil R. McEwan

Barbara Kowalik

Tadeusz Michałowski

Renata Miltko



Abstract

This study aimed to quantify the engulfed starch and reserve {esc}ga{esc}s-glucans (glycogen) in the cells of the ciliates Eudiplodinium maggii, as well the {esc}ga{esc}s-glucans in defaunated and selectively faunated sheep. The content of starch inside the cell of ciliates varied from 21 to 183 mg/g protozoal DM relative to the rumen fauna composition whereas, the glycogen fluctuated between 17 and 126 mg/g dry matter (DM) of this ciliate species. Establishment of the population Entodinium caudatum in the rumen of sheep already faunated with E. maggii caused a drop in both types of quantified carbohydrates. The content of {esc}ga{esc}s-glucans in the rumen of defaunated sheep varied from 4.4 to 19.9 mg/g DM and increased to 7.4-29.9 or 11.8-33.9 mg/g DM of rumen contents in the presence of only E. maggii or E. maggii and E. caudatum, respectively. The lowest content of the carbohydrates was always found just before feeding and the highest at 4h thereafter. The {esc}ga{esc}s-glucans in the reticulum varied 7.5-40.1, 14.3-76.8 or 21.9-106.1 mg/g DM of reticulum content for defaunated, monofaunated or bifaunated sheep, respectively. The results indicated that both ciliate species engulf starch granules and convert the digestion products to the glycogen, diminishing the pool of starch available for amylolytic bacteria.

Journal Article Type Article
Publication Date Feb 28, 2017
Journal European journal of protistology
Print ISSN 0932-4739
Electronic ISSN 1618-0429
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 57
Issue 38-49
Institution Citation BELZECKI, G., MCEWAN, N.R., KOWALIK, B., MICHALOWSKI, T. and MILTKO, R. 2017. Effect of Entodinium caudatum on starch intake and glycogen formation by Eudiplodinium maggii in the rumen and reticulum. European journal of protistology [online], 57, pages 38-49. Available from: https://doi.org/10.1016/j.ejop.2016.09.007
DOI https://doi.org/10.1016/j.ejop.2016.09.007
Keywords Engulfment; a-glucans; Glycogen; Rumen protozoa; Starch

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