Skip to main content

Research Repository

Advanced Search

Development of new silver nanoparticles suitable for materials with antimicrobial properties.

Ruttkay-Nedecky, Branislav; Skalickova, Sylvie; Kepinska, Marta; Cihalova, Kristyna; Docekalova, Michaela; Stankova, Martina; Uhlirova, Dagmar; Fernandez, Carlos; Sochor, Jiri; Milnerowicz, Halina; Beklova, Miroslava; Kizek, Rene


Branislav Ruttkay-Nedecky

Sylvie Skalickova

Marta Kepinska

Kristyna Cihalova

Michaela Docekalova

Martina Stankova

Dagmar Uhlirova

Jiri Sochor

Halina Milnerowicz

Miroslava Beklova

Rene Kizek


Silver nanoparticles are the most important nanoparticles in connection with the antimicrobial effect. Nowadays, the green synthesis of various types of nanoparticles is rapid, effective and produce less toxic nanoparticles often with specific properties. In our experiment we have developed and described in details various types of silver nanoparticles synthesized chemically or by the green synthesis. Nine different silver nanoparticles were synthesized, three by citrate method at different pHs (8; 9; 10), four using gallic acid at alkaline pHs (10; 11), and two by green synthesis using green tea and coffee extracts, both at pH 9. Characterisation of silver nanoparticles was performed using dynamic light scattering, scanning electron microscopy, and ultraviolet-visible absorption spectroscopy. Silver nanoparticles prepared by green synthesis showed the highest antioxidant activity and also ability for quenching of free radicals. Antibacterial activity of silver nanoparticles was determined on bacterial cultures such as Staphylococcus aureus and Escherichia coli. Silver nanoparticles synthesized using green tea and coffee extracts showed the highest antibacterial activity for both bacterial strains. Minimal inhibition concentration for both strains was found to be 65 {aelig}M at each silver nanoparticle synthesized using green synthesis.


RUTTKAY-NEDECKY, B., SKALICKOVA, S., KEPINSKA, M., CIHALOVA, K., DOCEKALOVA, M., STANKOVA, M., UHLIROVA, D., FERNANDEZ, C., SOCHOR, J., MILNEROWICZ, H., BEKLOVA, M. and KIZEK, R. 2019. Development of new silver nanoparticles suitable for materials with antimicrobial properties. Journal of nanoscience and nanotechnology [online], 19(5), pages 2762-2769. Available from:

Journal Article Type Article
Acceptance Date Sep 30, 2017
Online Publication Date Dec 18, 2018
Publication Date May 31, 2019
Deposit Date May 21, 2018
Publicly Available Date Dec 18, 2018
Journal Journal of nanoscience and nanotechnology
Print ISSN 1533-4880
Electronic ISSN 1533-4899
Publisher American Scientific Publishers
Peer Reviewed Peer Reviewed
Volume 19
Issue 5
Pages 2762-2769
Keywords Silver nanoparticles; Antioxidant activity; Antimicrobial activity; Green synthesis
Public URL