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Determination of sinapine in rapeseed pomace extract: its antioxidant and acetylcholinesterase inhibition properties.

Yates, Kyari; Pohl, Franziska; Busch, Maike; Mozer, Annika; Watters, Louis; Shiryaev, Andrey; Kong Thoo Lin, Paul

Authors

Franziska Pohl

Maike Busch

Annika Mozer

Louis Watters

Andrey Shiryaev



Abstract

Sinapine is the main secondary metabolite present in rapeseed pomace (RSP) with its concentration being dependent on rapeseed processing, growing conditions, extraction parameters and the country of origin. Here we report, the concentration of sinapine from an extract of defatted RSP harvested in the North East of Scotland. Using liquid chromatography tandem mass spectrometry, the most abundant phenolic compound in the RSP extract was, as expected, sinapine (109.1 mg/g RSP extract). Additionally, sinapic, caffeic, ferulic and syringic acids were identified (0.159-3.91 mg/g RSP extract). Sinapine together with the phenolics at the concentration present in the RSP extract, exhibited ≥ 50% activity relative to the extract in antioxidant assays. Furthermore, sinapine provided plasmid DNA (pBR322) protection, from 2,2'-azobis(2-amidinopropane) dihydrochloride and inhibited acetylcholinesterase activity by 85 %. Molecular docking was utilised to explain the inhibitory activity. RSP can be an excellent source of bioactive compounds for pharmaceuticals, food additive and nutraceutical applications.

Citation

YATES, K., POHL, F., BUSCH, M., MOZER, A., WATTERS, L., SHIRYAEV, A. and KONG THOO LIN, P. 2019. Determination of sinapine in rapeseed pomace extract: its antioxidant and acetylcholinesterase inhibition properties. Food chemistry [online], 276, pages 768-775. Available from: https://doi.org/10.1016/j.foodchem.2018.10.045

Journal Article Type Article
Acceptance Date Oct 9, 2018
Online Publication Date Oct 10, 2018
Publication Date Mar 15, 2019
Deposit Date Oct 15, 2018
Publicly Available Date Oct 11, 2019
Journal Food chemistry
Print ISSN 0308-8146
Electronic ISSN 1873-7072
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 276
Pages 768-775
DOI https://doi.org/10.1016/j.foodchem.2018.10.045
Keywords Rapeseed pomace; Canola; Sinapine; Phenolic acids; Antioxidant assays; Plasmid DNA (pBR322); LCMS/MS; Acetylcholinesterase (AChE)
Public URL http://hdl.handle.net/10059/3167

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