Determination of sinapine in rapeseed pomace extract: its antioxidant and acetylcholinesterase inhibition properties.

Yates, Kyari; Pohl, Franziska; Busch, Maike; Mozer, Annika; Watters, Louis; Shiryaev, Andrey; Kong Thoo Lin, Paul

Abstract

Sinapine is the main secondary metabolite present in rapeseed pomace (RSP) with its concentration being dependent on rapeseed processing, growing conditions, extraction parameters and the country of origin. Here we report, the concentration of sinapine from an extract of defatted RSP harvested in the North East of Scotland. Using liquid chromatography tandem mass spectrometry, the most abundant phenolic compound in the RSP extract was, as expected, sinapine (109.1 mg/g RSP extract). Additionally, sinapic, caffeic, ferulic and syringic acids were identified (0.159-3.91 mg/g RSP extract). Sinapine together with the phenolics at the concentration present in the RSP extract, exhibited ≥ 50% activity relative to the extract in antioxidant assays. Furthermore, sinapine provided plasmid DNA (pBR322) protection, from 2,2'-azobis(2-amidinopropane) dihydrochloride and inhibited acetylcholinesterase activity by 85 %. Molecular docking was utilised to explain the inhibitory activity. RSP can be an excellent source of bioactive compounds for pharmaceuticals, food additive and nutraceutical applications.

Citation

YATES, K., POHL, F., BUSCH, M., MOZER, A., WATTERS, L., SHIRYAEV, A. and KONG THOO LIN, P. 2019. Determination of sinapine in rapeseed pomace extract: its antioxidant and acetylcholinesterase inhibition properties. Food chemistry [online], 276, pages 768-775. Available from: https://doi.org/10.1016/j.foodchem.2018.10.045

• Journal Article Type: Article
• Acceptance Date: Oct 9, 2018
• Online Publication Date: Oct 10, 2018
• Publication Date: Mar 15, 2019
• Deposit Date: Oct 15, 2018
• Publicly Available Date: Oct 11, 2019
• Journal: Food chemistry
• Print ISSN: 0308-8146
• Electronic ISSN: 1873-7072
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 276
• Pages: 768-775
• DOI: https://doi.org/10.1016/j.foodchem.2018.10.045
• Keywords: Rapeseed pomace; Canola; Sinapine; Phenolic acids; Antioxidant assays; Plasmid DNA (pBR322); LCMS/MS; Acetylcholinesterase (AChE)
• Public URL: http://hdl.handle.net/10059/3167
• Contract Date: Oct 15, 2018