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Cytochrome P450 CYP3A in human renal cell cancer.

Murray, G.I.; McFadyen, M.C.E.; Mitchell, R.T.; Cheung, Y.-L.; Kerr, A.C.; Melvin, W.T.


G.I. Murray

M.C.E. McFadyen

R.T. Mitchell

Y.-L. Cheung

A.C. Kerr

W.T. Melvin


Renal cell cancer is the main malignant tumour of the kidney and has an increasing incidence. This type of tumour has a poor prognosis and shows intrinsic resistance to several anti-cancer drugs. The CYP3A P450 family, which consists of three closely related forms, is involved in the oxidative activation and deactivation of a variety of carcinogens and several anti-cancer drugs. In this study the presence and cellular localization of CYP3A has been investigated using a combination of immunohistochemistry, immunoblotting and reverse transcriptase polymerase chain reaction (RT-PCR) in renal cell cancer and corresponding normal kidney. CYP3A was consistently expressed in both renal call cancer and in normal kidney. In renal cell cancer, CYP3A was localized to tumour cells and in normal kidney the predominant cellular localization of CYP3A was to proximal tubular epithelial cells. RT-PCR showed that both CYP3A5 mRNA and CYP3A7 mRNA were consistently present in both tumour and normal samples, while CYP3A4 mRNA was present in 65% of tumours and 90% of normal samples. This study indicates that individual members of the CYP3A family are expressed in renal cell cancer. The presence of CYP3A in renal cell cancer might be important in the metabolic potentiation as well as the detoxification of chemotherapeutic agents used to renal cancer.


MURRAY, G.I., MCFADYEN, M.C.E., MITCHELL, R.T., CHEUNG, Y.-L., KERR, A.C. and MELVIN, W.T. 1999. Cytochrome P450 CYP3A in human renal cell cancer. British journal of cancer [online], 79(11-12), pages 1836-1842. Available from:

Journal Article Type Article
Acceptance Date Aug 26, 1998
Online Publication Date Mar 12, 1999
Publication Date Apr 1, 1999
Deposit Date Sep 4, 2020
Publicly Available Date Sep 4, 2020
Journal British journal of cancer
Print ISSN 0007-0920
Electronic ISSN 1532-1827
Publisher Springer
Peer Reviewed Peer Reviewed
Volume 79
Issue 11-12
Pages 1836-1842
Keywords Cytochrome P450; Kidney cancer; Neoplasm
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