In vitro and in vivo antimalarial activity assays of seeds from Balanites aegyptiaca: compounds of the extract show growth inhibition and activity against Plasmodial aminopeptidase.

Kusch, Peter; Deininger, Susanne; Specht, Sabine; Maniako, Rudeka; Haubrich, Stefanie; Pommerening, Tanja; Kong Thoo Lin, Paul; Hoerauf, Achim; Kaiser, Annette

Abstract

Balanites aegyptiaca (Balanitaceae) is a widely grown desert plant with multiuse potential. In the present paper, a crude extract from B. aegyptiaca seeds equivalent to a ratio of 1:2000 seeds to the extract was screened for antiplasmodial activity. The determined IC50 value for the chloroquine-susceptible Plasmodium falciparum NF54 strain was 68.26 μg/μL ± 3.5. Analysis of the extract by gas chromatography-mass spectrometry detected 6-phenyl-2(H)-1,2,4-triazin-5-one oxime, an inhibitor of the parasitic M18 Aspartyl Aminopeptidase as one of the compounds which is responsible for the in vitro antiplasmodial activity. The crude plant extract had a Ki of 2.35 μg/μL and showed a dose-dependent response. After depletion of the compound, a significantly lower inhibition was determined with a Ki of 4.8 μg/μL. Moreover, two phenolic compounds, that is, 2,6-di-tert-butyl-phenol and 2,4-di-tert-butyl-phenol, with determined IC50 values of 50.29 μM ± 3 and 47.82 μM ± 2.5, respectively, were detected. These compounds may contribute to the in vitro antimalarial activity due to their antioxidative properties. In an in vivo experiment, treatment of BALB/c mice with the aqueous Balanite extract did not lead to eradication of the parasites, although a reduced parasitemia at day 12 p.i. was observed.

Citation

KUSCH, P., DEININGER, S., SPECHT, S., MANIAKO, R., HAUBRICH, S., POMMERENING, T., KONG THOO LIN, P., HOERAUF, A. and KAISER, A. 2011. In vitro and in vivo antimalarial activity assays of seeds from Balanites aegyptiaca: compounds of the extract show growth inhibition and activity against Plasmodial aminopeptidase. Journal of parasitology research [online], 2011, article ID 368692. Available from: https://doi.org/10.1155/2011/368692

• Journal Article Type: Article
• Acceptance Date: Mar 20, 2011
• Online Publication Date: May 25, 2011
• Publication Date: Dec 31, 2011
• Deposit Date: Aug 23, 2022
• Publicly Available Date: Sep 5, 2022
• Journal: Journal of Parasitology Research
• Print ISSN: 2090-0023
• Electronic ISSN: 2090-0031
• Publisher: Hindawi
• Peer Reviewed: Peer Reviewed
• Volume: 2011
• Article Number: 368692
• DOI: https://doi.org/10.1155/2011/368692
• Keywords: Balanites aegyptiaca; Crude plant extract; Phenolic compunds; Antimalarial activity; Antioxidative properties
• Public URL: https://rgu-repository.worktribe.com/output/865501