Synthesis of oligodeoxyribonucleotides containing degenerate bases and their use as primers in the polymerase chain reaction.

Kong Thoo Lin, Paul; Brown, Daniel M.

Abstract

Heptadecaoligodeoxyribonudeotides containing one or more of the bases, 6H,8H-3,4-dihydropyrimido[4,5-c] [1,2]oxazin-7-one (P), 2-amino-6-methoxyaminopurine (K), and hypoxanthine (I) and combinations of P with K and I have been synthesised on a DNA synthesiser. The stability of duplexes containing these base-modified oligomers with P/A, P/G, K/C and K/T; P/A, P/G, I/C, I/T and I/A, I/G, I/C, I/T base pairs were compared by measuring their melting transition (T m ) values. Oligomers containing both P and K and P and I were more stable than those with I alone or with mismatches. These oligomers together with one with a P base at the 3'-end were used as primers in polymerase chain reaction (PCR) experiments. They were all effective primers except one with I alone and a triple mismatch. Thus the use of the degenerate bases P and K in primer design is established.

Citation

KONG THOO LIN, P. and BROWN, D.M. 1992. Synthesis of oligodeoxyribonucleotides containing degenerate bases and their use as primers in the polymerase chain reaction. Nucleic acids research [online], 20(19), pages 5149-5152. Available from: https://doi.org/10.1093/nar/20.19.5149

• Journal Article Type: Article
• Acceptance Date: Sep 11, 1992
• Online Publication Date: Oct 11, 1992
• Publication Date: Oct 11, 1992
• Deposit Date: Aug 23, 2022
• Publicly Available Date: Dec 16, 2022
• Journal: Nucleic Acids Research
• Print ISSN: 0305-1048
• Electronic ISSN: 1362-4962
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 20
• Issue: 19
• Pages: 5149-5152
• DOI: https://doi.org/10.1093/nar/20.19.5149
• Keywords: Synthesis of monomers; Degenerate bases; DNA duplexes
• Public URL: https://rgu-repository.worktribe.com/output/866421