Synthesis and duplex stability of oligonucleotides containing cytosine-thymine analogues.

Kong Thoo Lin, P.; Brown, Daniel M.

Abstract

The synthesis of the deoxynucleoside derived from the base P, 6H, 8H-3, 4-dihydro-pyrimido[4, 5-c][1, 2] oxazin-7-one, 2, and its introduction by established phosphoramidite and H-phosphonate chemistry into oligonucleotides is described. The melting transition temperatures (Tm ) of a range of heptadecamer duplexes containing P/A and P/G base-pairs are compared with corresponding ones having N4 -methoxycytosine (M) 1 and mismatched normal bases. P/A and P/G pairs allow closely similar duplex stabilities and have the potential to reduce the multiplicity of probes and primers based on amino acid sequences by removing the T/C degeneracy.

Citation

KONG THOO LIN, P. and BROWN, D.M. 1989. Synthesis and duplex stability of oligonucleotides containing cytosine-thymine analogues. Nucleic acids research [online], 17(24), pages 10373-10383. Available from: https://doi.org/10.1093/nar/17.24.10373

• Journal Article Type: Article
• Acceptance Date: Nov 10, 1989
• Online Publication Date: Dec 25, 1989
• Publication Date: Dec 25, 1989
• Deposit Date: Aug 23, 2022
• Publicly Available Date: Mar 28, 2023
• Journal: Nucleic Acids Research
• Print ISSN: 0305-1048
• Electronic ISSN: 1362-4962
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 17
• Issue: 24
• Pages: 10373-10383
• DOI: https://doi.org/10.1093/nar/17.24.10373
• Keywords: Amino acids; DNA; RNA; Oligonucleotides
• Public URL: https://rgu-repository.worktribe.com/output/866518