The hen’s egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: quantification using Photoshop® and ImageJ.

McKenzie, Barbara; Kay, Graeme; Matthews, Kerr H.; Knott, Rachel M.; Cairns, Donald

Abstract

A modified hen’s egg chorioallantoic membrane (HET-CAM) test has been developed, combining ImageJ analysis with Adobe® Photoshop®. The irritation potential of an ophthalmic medicine can be quantified using this method, by monitoring damage to blood vessels. The evaluation of cysteamine containing hyaluronate gel is reported. The results demonstrated that the novel gel formulation is non-irritant to the ocular tissues, in line with saline solution (negative control). In conclusion, the modification of the established HET-CAM test can quantify the damage to minute blood vessels. These results offer the possibility to formulate cysteamine in an ocular applicable gel formulation.

Citation

MCKENZIE, B., KAY, G., MATTHEWS, K.H., KNOTT, R.M. and CAIRNS, D. 2015. The hen’s egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: quantification using Photoshop® and ImageJ. International journal of pharmaceutics [online], 490(1-2), pages 1-8. Available from: https://doi.org/10.1016/j.ijpharm.2015.05.023

• Journal Article Type: Article
• Acceptance Date: May 7, 2015
• Online Publication Date: May 13, 2015
• Publication Date: Jul 25, 2015
• Deposit Date: Mar 3, 2020
• Publicly Available Date: Mar 3, 2020
• Journal: International Journal of Pharmaceutics
• Print ISSN: 0378-5173
• Electronic ISSN: 1873-3476
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 490
• Issue: 1-2
• Pages: 1-8
• DOI: https://doi.org/10.1016/j.ijpharm.2015.05.023
• Keywords: HET-CAM test; ImageJ; Photoshop®; Ophthalmic; Cystinosis; Cysteamine
• Public URL: https://rgu-repository.worktribe.com/output/841849