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The hen’s egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: quantification using Photoshop® and ImageJ.

McKenzie, Barbara; Kay, Graeme; Matthews, Kerr H.; Knott, Rachel M.; Cairns, Donald

Authors

Barbara McKenzie

Graeme Kay

Kerr H. Matthews

Rachel M. Knott

Donald Cairns



Abstract

A modified hen’s egg chorioallantoic membrane (HET-CAM) test has been developed, combining ImageJ analysis with Adobe® Photoshop®. The irritation potential of an ophthalmic medicine can be quantified using this method, by monitoring damage to blood vessels. The evaluation of cysteamine containing hyaluronate gel is reported. The results demonstrated that the novel gel formulation is non-irritant to the ocular tissues, in line with saline solution (negative control). In conclusion, the modification of the established HET-CAM test can quantify the damage to minute blood vessels. These results offer the possibility to formulate cysteamine in an ocular applicable gel formulation.

Journal Article Type Article
Publication Date Jul 25, 2015
Journal International journal of pharmaceutics
Print ISSN 0378-5173
Electronic ISSN 1873-3476
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 490
Issue 1-2
Pages 1-8
Institution Citation MCKENZIE, B., KAY, G., MATTHEWS, K.H., KNOTT, R.M. and CAIRNS, D. 2015. The hen’s egg chorioallantoic membrane (HET-CAM) test to predict the ophthalmic irritation potential of a cysteamine-containing gel: quantification using Photoshop® and ImageJ. International journal of pharmaceutics [online], 490(1-2), pages 1-8. Available from: https://doi.org/10.1016/j.ijpharm.2015.05.023
DOI https://doi.org/10.1016/j.ijpharm.2015.05.023
Keywords HET-CAM test; ImageJ; Photoshop®; Ophthalmic; Cystinosis; Cysteamine

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